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BMRB: 19095

2MNA

Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single-stranded DNA binding protein from Sulfolobus Solfataricus

Authors

Gamsjaeger, R., Kariawasam, R., Touma, C., White, M.F., Cubeddu, L.

Assembly

SsoSSB

Entity

1. SsoSSB (polymer, Thiol state: not present), 117 monomers, 12761.22 Da Detail

MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTARRR

Formula weight

12761.22 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing, torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.6 % (1311 of 1330)	98.0 % (682 of 696)	99.2 % (502 of 506)	99.2 % (127 of 128)
Backbone	98.7 % (685 of 694)	97.9 % (235 of 240)	99.1 % (338 of 341)	99.1 % (112 of 113)
Sidechain	98.5 % (732 of 743)	98.0 % (447 of 456)	99.3 % (270 of 272)	100.0 % (15 of 15)
Aromatic	97.9 % (47 of 48)	100.0 % (24 of 24)	95.5 % (21 of 22)	100.0 % (2 of 2)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. SsoSSB

MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTARRR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SsoSSB	[U-100% 13C; U-100% 15N]	0.8 ~ 1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MNA, Strand ID: A Detail

Release date

2013-04-16

Citation

Backbone and side-chain ¹H, ¹³C and ¹⁵N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface
Gamsjaeger, R., Kariawasam, R., Touma, C., Kwan, A.H., White, M.F., Cubeddu, L.
Biomol. NMR Assign. (2014), 8, 243-246, PubMed 23749431 , DOI 10.1007/s12104-013-9492-4 , Abstract

Related entities 1. SsoSSB, : 1 : 3 : 18 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC