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Found 1 Document (0.891 seconds)

BMRB: 19406

2MBO

K11-linked Diubiquitin average solution structure at pH 6.8, 0 mM NaCl

Authors

Castaneda, C.A., Fushman, D.

Assembly

K11-linked Diubiquitin

Entity

1. K11-linked Diubiquitin (polymer, Thiol state: not present), 152 monomers, 17111.45 × 2 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GG

Total weight

34222.9 Da

Max. entity weight

17111.45 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

in-house RDC (orientation) and MTSL (distance)

Data set

assigned_chemical_shifts

Chemical Shifts 1

¹H: 70, ¹⁵N: 70 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val
1	1	2	GLN	H	H	8.871
2	1	2	GLN	N	N	122.891
3	1	3	ILE	H	H	8.289
4	1	3	ILE	N	N	115.103
5	1	4	PHE	H	H	8.588
6	1	4	PHE	N	N	118.739
7	1	5	VAL	H	H	9.273
8	1	5	VAL	N	N	121.437
9	1	6	LYS	H	H	9.019
10	1	6	LYS	N	N	128.204
11	1	7	THR	H	H	8.651
12	1	7	THR	N	N	115.626
13	1	8	LEU	H	H	9.116
14	1	8	LEU	N	N	121.501
15	1	9	THR	H	H	7.639
16	1	9	THR	N	N	105.890
17	1	10	GLY	H	H	7.858
18	1	10	GLY	N	N	109.511
19	1	11	LYS	H	H	7.243
20	1	11	LYS	N	N	121.448
21	1	12	THR	H	H	8.674
22	1	12	THR	N	N	120.346
23	1	13	ILE	H	H	9.496
24	1	13	ILE	N	N	127.439
25	1	14	THR	H	H	8.713
26	1	14	THR	N	N	121.401
27	1	15	LEU	H	H	8.702
28	1	15	LEU	N	N	125.182
29	1	16	GLU	H	H	8.111
30	1	16	GLU	N	N	122.667
31	1	17	VAL	H	H	8.921
32	1	17	VAL	N	N	117.681
33	1	18	GLU	H	H	8.630
34	1	18	GLU	N	N	119.392
35	1	20	SER	H	H	7.012
36	1	20	SER	N	N	103.536
37	1	21	ASP	H	H	8.032
38	1	21	ASP	N	N	124.088
39	1	22	THR	H	H	7.882
40	1	22	THR	N	N	109.196
41	1	23	ILE	H	H	8.502
42	1	23	ILE	N	N	121.357
43	1	25	ASN	H	H	7.915
44	1	25	ASN	N	N	121.633
45	1	26	VAL	H	H	8.104
46	1	26	VAL	N	N	122.347
47	1	27	LYS	H	H	8.558
48	1	27	LYS	N	N	119.125
49	1	28	ALA	H	H	7.964
50	1	28	ALA	N	N	123.696
51	1	29	LYS	H	H	7.842
52	1	29	LYS	N	N	120.455
53	1	30	ILE	H	H	8.282
54	1	30	ILE	N	N	121.462
55	1	31	GLN	H	H	8.567
56	1	31	GLN	N	N	123.771
57	1	32	ASP	H	H	7.992
58	1	32	ASP	N	N	119.854
59	1	33	LYS	H	H	7.392
60	1	33	LYS	N	N	115.518
61	1	34	GLU	H	H	8.749
62	1	34	GLU	N	N	114.369
63	1	35	GLY	H	H	8.510
64	1	35	GLY	N	N	108.982
65	1	36	ILE	H	H	6.143
66	1	36	ILE	N	N	120.148
67	1	39	ASP	H	H	8.524
68	1	39	ASP	N	N	113.773
69	1	40	GLN	H	H	7.822
70	1	40	GLN	N	N	117.059
71	1	41	GLN	H	H	7.476
72	1	41	GLN	N	N	118.178
73	1	42	ARG	H	H	8.490
74	1	42	ARG	N	N	123.067
75	1	43	LEU	H	H	8.806
76	1	43	LEU	N	N	124.430
77	1	44	ILE	H	H	9.107
78	1	44	ILE	N	N	122.600
79	1	45	PHE	H	H	8.846
80	1	45	PHE	N	N	125.181
81	1	46	ALA	H	H	9.006
82	1	46	ALA	N	N	133.188
83	1	47	GLY	H	H	8.108
84	1	47	GLY	N	N	102.588
85	1	48	LYS	H	H	7.951
86	1	48	LYS	N	N	122.079
87	1	49	GLN	H	H	8.657
88	1	49	GLN	N	N	123.318
89	1	50	LEU	H	H	8.550
90	1	50	LEU	N	N	125.842
91	1	51	GLU	H	H	8.380
92	1	51	GLU	N	N	123.246
93	1	52	ASP	H	H	8.156
94	1	52	ASP	N	N	120.540
95	1	54	ARG	H	H	7.434
96	1	54	ARG	N	N	119.473
97	1	55	THR	H	H	8.820
98	1	55	THR	N	N	108.991
99	1	56	LEU	H	H	8.142
100	1	56	LEU	N	N	118.142
101	1	57	SER	H	H	8.457
102	1	57	SER	N	N	113.648
103	1	58	ASP	H	H	7.916
104	1	58	ASP	N	N	124.663
105	1	59	TYR	H	H	7.244
106	1	59	TYR	N	N	115.916
107	1	60	ASN	H	H	8.133
108	1	60	ASN	N	N	116.069
109	1	61	ILE	H	H	7.235
110	1	61	ILE	N	N	119.135
111	1	62	GLN	H	H	7.635
112	1	62	GLN	N	N	125.060
113	1	63	LYS	H	H	8.472
114	1	63	LYS	N	N	120.635
115	1	64	GLU	H	H	9.297
116	1	64	GLU	N	N	115.322
117	1	65	SER	H	H	7.719
118	1	65	SER	N	N	115.170
119	1	66	THR	H	H	8.693
120	1	66	THR	N	N	117.501
121	1	67	LEU	H	H	9.386
122	1	67	LEU	N	N	127.946
123	1	68	HIS	H	H	9.233
124	1	68	HIS	N	N	119.768
125	1	69	LEU	H	H	8.252
126	1	69	LEU	N	N	123.923
127	1	70	VAL	H	H	9.172
128	1	70	VAL	N	N	127.149
129	1	71	LEU	H	H	8.133
130	1	71	LEU	N	N	123.402
131	1	72	ARG	H	H	8.564
132	1	72	ARG	N	N	123.435
133	1	73	LEU	H	H	8.357
134	1	73	LEU	N	N	124.023
135	1	74	ARG	H	H	8.518
136	1	74	ARG	N	N	121.993
137	1	75	GLY	H	H	8.546
138	1	75	GLY	N	N	110.520
139	1	76	GLY	H	H	8.380
140	1	76	GLY	N	N	108.997

Chemical Shifts 2

¹H: 70, ¹⁵N: 70 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val
141	1	2	GLN	H	H	8.891
142	1	2	GLN	N	N	123.127
143	1	3	ILE	H	H	8.285
144	1	3	ILE	N	N	115.197
145	1	4	PHE	H	H	8.570
146	1	4	PHE	N	N	118.586
147	1	5	VAL	H	H	9.273
148	1	5	VAL	N	N	121.668
149	1	6	LYS	H	H	8.957
150	1	6	LYS	N	N	127.765
151	1	7	THR	H	H	8.670
152	1	7	THR	N	N	114.686
153	1	8	LEU	H	H	9.167
154	1	8	LEU	N	N	121.436
155	1	9	THR	H	H	7.599
156	1	9	THR	N	N	105.794
157	1	10	GLY	H	H	7.757
158	1	10	GLY	N	N	109.049
159	1	11	LYS	H	H	7.260
160	1	11	LYS	N	N	122.265
161	1	12	THR	H	H	8.547
162	1	12	THR	N	N	119.932
163	1	13	ILE	H	H	9.543
164	1	13	ILE	N	N	127.779
165	1	14	THR	H	H	8.739
166	1	14	THR	N	N	121.893
167	1	15	LEU	H	H	8.662
168	1	15	LEU	N	N	125.148
169	1	16	GLU	H	H	8.102
170	1	16	GLU	N	N	122.662
171	1	17	VAL	H	H	8.930
172	1	17	VAL	N	N	117.747
173	1	18	GLU	H	H	8.622
174	1	18	GLU	N	N	119.378
175	1	20	SER	H	H	7.007
176	1	20	SER	N	N	103.499
177	1	21	ASP	H	H	8.035
178	1	21	ASP	N	N	124.124
179	1	22	THR	H	H	7.883
180	1	22	THR	N	N	109.205
181	1	23	ILE	H	H	8.498
182	1	23	ILE	N	N	121.454
183	1	25	ASN	H	H	7.912
184	1	25	ASN	N	N	121.521
185	1	26	VAL	H	H	8.116
186	1	26	VAL	N	N	122.398
187	1	27	LYS	H	H	8.504
188	1	27	LYS	N	N	119.074
189	1	28	ALA	H	H	7.959
190	1	28	ALA	N	N	123.463
191	1	29	LYS	H	H	7.924
192	1	29	LYS	N	N	120.676
193	1	30	ILE	H	H	8.256
194	1	30	ILE	N	N	121.667
195	1	31	GLN	H	H	8.467
196	1	31	GLN	N	N	123.586
197	1	32	ASP	H	H	8.116
198	1	32	ASP	N	N	120.145
199	1	33	LYS	H	H	7.522
200	1	33	LYS	N	N	115.659
201	1	34	GLU	H	H	8.605
202	1	34	GLU	N	N	113.560
203	1	35	GLY	H	H	8.501
204	1	35	GLY	N	N	109.248
205	1	36	ILE	H	H	6.128
206	1	36	ILE	N	N	120.288
207	1	39	ASP	H	H	8.523
208	1	39	ASP	N	N	113.836
209	1	40	GLN	H	H	7.763
210	1	40	GLN	N	N	117.038
211	1	41	GLN	H	H	7.463
212	1	41	GLN	N	N	117.981
213	1	42	ARG	H	H	8.457
214	1	42	ARG	N	N	123.211
215	1	43	LEU	H	H	8.783
216	1	43	LEU	N	N	124.658
217	1	44	ILE	H	H	9.146
218	1	44	ILE	N	N	122.655
219	1	45	PHE	H	H	8.810
220	1	45	PHE	N	N	125.000
221	1	46	ALA	H	H	8.981
222	1	46	ALA	N	N	133.171
223	1	47	GLY	H	H	8.112
224	1	47	GLY	N	N	102.612
225	1	48	LYS	H	H	7.935
226	1	48	LYS	N	N	122.171
227	1	49	GLN	H	H	8.650
228	1	49	GLN	N	N	123.368
229	1	50	LEU	H	H	8.558
230	1	50	LEU	N	N	125.850
231	1	51	GLU	H	H	8.385
232	1	51	GLU	N	N	123.293
233	1	52	ASP	H	H	8.168
234	1	52	ASP	N	N	120.597
235	1	54	ARG	H	H	7.445
236	1	54	ARG	N	N	119.536
237	1	55	THR	H	H	8.801
238	1	55	THR	N	N	108.885
239	1	56	LEU	H	H	8.133
240	1	56	LEU	N	N	118.147
241	1	57	SER	H	H	8.473
242	1	57	SER	N	N	113.649
243	1	58	ASP	H	H	7.918
244	1	58	ASP	N	N	124.680
245	1	59	TYR	H	H	7.239
246	1	59	TYR	N	N	115.918
247	1	60	ASN	H	H	8.131
248	1	60	ASN	N	N	116.053
249	1	61	ILE	H	H	7.231
250	1	61	ILE	N	N	119.155
251	1	62	GLN	H	H	7.631
252	1	62	GLN	N	N	125.025
253	1	63	LYS	H	H	8.472
254	1	63	LYS	N	N	120.627
255	1	64	GLU	H	H	9.295
256	1	64	GLU	N	N	115.394
257	1	65	SER	H	H	7.727
258	1	65	SER	N	N	115.181
259	1	66	THR	H	H	8.683
260	1	66	THR	N	N	117.453
261	1	67	LEU	H	H	9.399
262	1	67	LEU	N	N	128.042
263	1	68	HIS	H	H	9.202
264	1	68	HIS	N	N	119.825
265	1	69	LEU	H	H	8.317
266	1	69	LEU	N	N	123.987
267	1	70	VAL	H	H	9.183
268	1	70	VAL	N	N	127.558
269	1	71	LEU	H	H	8.155
270	1	71	LEU	N	N	124.055
271	1	72	ARG	H	H	8.607
272	1	72	ARG	N	N	124.219
273	1	73	LEU	H	H	8.389
274	1	73	LEU	N	N	124.450
275	1	74	ARG	H	H	8.442
276	1	74	ARG	N	N	121.767
277	1	75	GLY	H	H	8.555
278	1	75	GLY	N	N	110.768
279	1	76	GLY	H	H	8.192
280	1	76	GLY	N	N	109.395

Protein Blocks Logo

Calculated from 1 models in PDB: 2MBO, Strand ID: A, B Detail

Release date

2013-08-15

Citation

Unique structural, dynamical, and functional properties of k11-linked polyubiquitin chains
Castaneda, C.A., Kashyap, T.R., Nakasone, M.A., Krueger, S., Fushman, D.
Structure (2013), 21, 1168-1181, PubMed 23823328 , DOI 10.1016/j.str.2013.04.029 , Abstract

Related entities 1. K11-linked Diubiquitin, : 203 : 6 : 85 : 226 entities Detail

Interaction partners 1. K11-linked Diubiquitin, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 2 experiments Detail

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 15N]	75 uM
2	sodium phosphate	natural abundance	20 mM
3	H2O	natural abundance	93 %
4	D2O	natural abundance	7 %

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 15N]	75 uM
2	sodium phosphate	natural abundance	20 mM
3	H2O	natural abundance	93 %
4	D2O	natural abundance	7 %

nullKeywords K11, diubiquitin, protein

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