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BMRB: 19428

2MC5

A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

Authors

Liu, B., Shadrin, A., Sheppard, C., Xu, Y., Severinov, K., Matthews, S., Wigneshweraraj, S.

Assembly

entity

Entity

1. entity (polymer, Thiol state: not present), 71 monomers, 7923.613 Da Detail

MNEFTQISGY VNAFGSQRGS VLTVKVENDE GWTLVEEDFD RADYGSDPEF VAEVSSYLKR NGGIKDLTKV L

Formula weight

7923.613 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 86.0 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.0 % (695 of 808)	84.6 % (352 of 416)	87.9 % (277 of 315)	85.7 % (66 of 77)
Backbone	92.0 % (390 of 424)	91.2 % (135 of 148)	92.2 % (190 of 206)	92.9 % (65 of 70)
Sidechain	81.7 % (366 of 448)	81.0 % (217 of 268)	85.5 % (148 of 173)	14.3 % (1 of 7)
Aromatic	73.7 % (56 of 76)	78.9 % (30 of 38)	70.3 % (26 of 37)	0.0 % (0 of 1)
Methyl	83.8 % (62 of 74)	83.8 % (31 of 37)	83.8 % (31 of 37)

1. entity

MNEFTQISGY VNAFGSQRGS VLTVKVENDE GWTLVEEDFD RADYGSDPEF VAEVSSYLKR NGGIKDLTKV L

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 8.7

#	Name	Isotope labeling	Concentration
1	entity	natural abundance	5 mM
2	sodium chloride	natural abundance	5 mM
3	potassium phosphate	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2MC5, Strand ID: A Detail

Release date

2014-02-11

Citation

A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement
Liu, B., Shadrin, A., Sheppard, C., Mekler, V., Xu, Y., Severinov, K., Matthews, S., Wigneshweraraj, S.
Nucleic Acids Res. (2014), 42, 4294-4305, PubMed 24482445 , DOI 10.1093/nar/gku080 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34024 released on 2017-08-07
    Title Gp5.7 mutant L42A
  BMRB: 19429 released on 2014-02-11
    Title A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

Related entities 1. entity, : 1 : 1 : 6 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC