BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	66.9 % (521 of 779)	70.6 % (290 of 411)	59.2 % (180 of 304)	79.7 % (51 of 64)
Backbone	74.6 % (288 of 386)	80.5 % (107 of 133)	68.8 % (132 of 192)	80.3 % (49 of 61)
Sidechain	57.6 % (261 of 453)	65.1 % (181 of 278)	45.3 % (78 of 172)	66.7 % (2 of 3)
Aromatic	42.1 % (16 of 38)	42.1 % (8 of 19)	38.9 % (7 of 18)	100.0 % (1 of 1)
Methyl	80.6 % (58 of 72)	83.3 % (30 of 36)	77.8 % (28 of 36)

1. HicA

DRTGSELMNS SKLIRMLEED GWRLVRVTGS AHHFKHPKKP GLVTVPHPKK DLPIGTVKSI QKSAGL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Concentration
3	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
4	NaCl	natural abundance	150.0 mM
5	sodium phosphate	natural abundance	20.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.47 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 4C26, Strand ID: A Detail

Release date

2014-02-11

Citation

The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation
Butt, A., Higman, V.A., Williams, C., Crump, M.P., Hemsley, C.M., Harmer, N., Titball, R.W.
Biochem. J. (2014), 459, 333-344, PubMed 24502667 , DOI 10.1042/BJ20140073 , Abstract

Related entities 1. HicA, : 1 : 1 : 2 entities Detail

Experiments performed 12 experiments Detail

1 3D 1H-15N NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

2 3D 1H-15N TOCSY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

3 HNHA (H{[N]+[HA]})

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

4 3D HNCA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

5 2D 1H-15N HSQC/HMQC

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

6 2D 1H-13C HSQC/HMQC

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

7 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

8 3D HNCO

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

9 3D HCCH-TOCSY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

10 2D 1H-1H NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.400

#	Name	Isotope labeling	Concentration
3	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
4	NaCl	natural abundance	150.0 mM
5	sodium phosphate	natural abundance	20.0 mM

11 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

12 HNHA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.400, Details HicA 1.0mM, NaCl 150mM, sodium phosphate 20mM

#	Name	Isotope labeling	Concentration
6	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
7	NaCl	natural abundance	150.0 mM
8	sodium phosphate	natural abundance	20.0 mM

nullKeywords HicA, toxin

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.718 seconds)

BMRB: 19464

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HicA, : 1 : 1 : 2 entities Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample