BMRBj - BMREntryMaster

Found 1 Document (0.504 seconds)

BMRB: 195

1PRA

Stereospecific Nuclear Magnetic Resonance Assignments of the Methyl Groups of Valine and Leucine in the DNA-Binding Domain of the 434 Repressor by Biosynthetically Directed Fractional 13C Labeling

Authors

Neri, D., Szyperski, T., Otting, G., Senn, H., Wuthrich, K.

Assembly

434 repressor

Entity

1. 434 repressor (polymer), 69 monomers, 7550.460 Da Detail

SISSRVKSKR IQLGLNQAEL AQKVGTTQQS IEQLENGKTK RPRFLPELAS ALGVSVDWLL NGTSDSNVR

Formula weight

7550.46 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 24.6 %, Completeness: 10.0 %, Completeness (bb): 4.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	10.0 % (72 of 721)	7.6 % (32 of 420)	13.3 % (40 of 301)
Backbone	4.1 % (14 of 343)	2.1 % (3 of 141)	5.4 % (11 of 202)
Sidechain	13.8 % (61 of 442)	10.4 % (29 of 279)	19.6 % (32 of 163)
Aromatic	0.0 % (0 of 21)	0.0 % (0 of 11)	0.0 % (0 of 10)
Methyl	69.0 % (58 of 84)	69.0 % (29 of 42)	69.0 % (29 of 42)

1. 434 repressor

SISSRVKSKR IQLGLNQAEL AQKVGTTQQS IEQLENGKTK RPRFLPELAS ALGVSVDWLL NGTSDSNVR

Show sample condition

Sample

Temperature 301 K, pH 6

Release date

1995-07-30

Citation

Stereospecific nuclear magnetic resonance assignments of the methyl groups of valine and leucine in the DNA-binding domain of the 434 repressor by biosynthetically directed fractional 13C labeling
Neri, D., Szyperski, T., Otting, G., Senn, H., Wuthrich, K.
Biochemistry (1989), 28, 7510-7516, PubMed 2692701 , DOI: , Abstract

Related entities 1. 434 repressor, : 1 : 5 : 2 : 34 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr195_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr195_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	112		20.3 (chain: 1, length: 69)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 69, Sequence coverage: 20.3%
- Total number of assignments
  - ¹³C chemical shifts: 28
  - ¹H chemical shifts: 84
- Completeness of assignments
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts

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Found 1 Document (0.504 seconds)

BMRB: 195

Sample

Related entities 1. 434 repressor, : 1 : 5 : 2 : 34 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail