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BMRB: 19610

2MH0

Solution NMR structure of the p300 Taz2:ETAD1 complex

Authors

Langelaan, D.N., Smith, S.P., Chitayat, S.

Assembly

p300 Taz2:ETAD1 complex

Entity

1. ETAD1 (polymer, Thiol state: not present), 39 monomers, 4263.892 Da Detail

GSMNQPQRMA PVGTDKELSD LLDFSMMFPL PVTNGKGRP

2. Taz2 (polymer), 92 monomers, 10087.75 Da Detail

GSATQSPGDS RRLSIQRAIQ SLVHAAQCRN ANCSLPSCQK MKRVVQHTKG CKRKTNGGCP ICKQLIALAA YHAKHCQENK CPVPFCLNIK QK

Total weight

14351.643 Da

Max. entity weight

10087.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.5 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.5 % (1411 of 1509)	89.9 % (721 of 802)	97.5 % (554 of 568)	97.8 % (136 of 139)
Backbone	96.6 % (740 of 766)	95.4 % (249 of 261)	97.1 % (373 of 384)	97.5 % (118 of 121)
Sidechain	91.9 % (795 of 865)	87.4 % (473 of 541)	99.3 % (304 of 306)	100.0 % (18 of 18)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)
Methyl	98.2 % (112 of 114)	96.5 % (55 of 57)	100.0 % (57 of 57)

1. ETAD1

GSMNQPQRMA PVGTDKELSD LLDFSMMFPL PVTNGKGRP

2. Taz2

GSATQSPGDS RRLSIQRAIQ SLVHAAQCRN ANCSLPSCQK MKRVVQHTKG CKRKTNGGCP ICKQLIALAA YHAKHCQENK CPVPFCLNIK QK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled Taz2 Unlabeled ETAD1 pH 6.5

#	Name	Isotope labeling	Concentration
8	ETAD1	natural abundance	3078 uM
9	Taz2	[U-99% 13C; U-99% 15N]	1038 uM
10	MES	natural abundance	20 mM
11	sodium azide	natural abundance	1 mM
12	beta-mercaptoethanol	natural abundance	5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.85 ppm	internal	indirect	0.2514495
¹H	water	protons	4.85 ppm	internal	direct	1.0
¹⁵N	water	protons	4.85 ppm	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.85 ppm	internal	indirect	0.2514495
¹H	water	protons	4.85 ppm	internal	direct	1.0
¹⁵N	water	protons	4.85 ppm	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.85 ppm	internal	indirect	0.2514495
¹H	water	protons	4.85 ppm	internal	direct	1.0
¹⁵N	water	protons	4.85 ppm	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.85 ppm	internal	indirect	0.2514495
¹H	water	protons	4.85 ppm	internal	direct	1.0
¹⁵N	water	protons	4.85 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MH0, Strand ID: A, B Detail

Release date

2014-01-08

Citation

CBP/p300 TAZ1 domain forms a structured scaffold for ligand binding
Lochhead, M.R., Brown, A.D., Kirlin, A.C., Chitayat, S., Munro, K., Findlay, J.E., Baillie, G.S., LeBrun, D.P., Langelaan, D.N., Smith, S.P.
Biochemistry (2005), 44, 490-497, PubMed 15641773 , DOI 10.1021/bi048161t , Abstract

Related entities 1. ETAD1, : 1 : 3 : 18 entities Detail

Related entities 2. Taz2, : 51 entities Detail

Interaction partners 1. ETAD1, : 31 interactors Detail

More details for 31 interactors identified by 12 citations

Interaction partners 2. Taz2, : 6 interactors Detail

More details for 6 interactors identified by 6 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HCACO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz QANUC

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 800 MHz QANUC

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 800 MHz QANUC

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details Labeled ETAD1, unlabeled Taz2 pH 6.5

#	Name	Isotope labeling	Concentration
1	ETAD1	[U-99% 13C; U-99% 15N]	1.4 mM
2	Taz2	natural abundance	2 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

10 2D 1H-15N HSQC