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BMRB: 19653

4CH1

RRM domain from C. elegans SUP-12

Authors

Amrane, S., Mackereth, C.D.

Assembly

RRM domain from C. elegans SUP-12 + GGTGTGC DNA

Entity

1. PROTEIN SUP-12 ISOFORM B (polymer, Thiol state: all free), 97 monomers, 10815.06 Da Detail

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

2. GGTGTGC (polymer, Thiol state: not present), 7 monomers, 2232.405 Da Detail

GGTGTGC

Total weight

13047.465 Da

Max. entity weight

10815.06 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

ARIA1.2

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.2 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	96.2 % (1133 of 1178)	95.8 % (619 of 646)	96.5 % (416 of 431)	97.0 % (98 of 101)
Backbone	96.1 % (599 of 623)	96.4 % (239 of 248)	95.7 % (270 of 282)	96.8 % (90 of 93)
Sidechain	96.6 % (621 of 643)	95.5 % (380 of 398)	98.3 % (233 of 237)	100.0 % (8 of 8)
Aromatic	91.3 % (95 of 104)	89.8 % (53 of 59)	93.3 % (42 of 45)
Methyl	98.9 % (93 of 94)	97.9 % (47 of 48)	100.0 % (46 of 46)

1. PROTEIN SUP-12 ISOFORM B

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

2. GGTGTGC

GGTGTGC

Show sample condition

Sample #1

Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.8mM

#	Name	Isotope labeling	Concentration
1	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.8 mM
2	GGTGTGC	natural abundance	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	10 %
6	D2O	[U-100% 2H]	90 %

Sample #2

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.4mM

#	Name	Isotope labeling	Concentration
7	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.4 mM
8	GGTGTGC	natural abundance	0.4 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	[U-100% 2H]	100 %

Sample #3

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.6mM

#	Name	Isotope labeling	Concentration
12	PROTEIN SUP-12, ISOFORM B	[U-100% 2H]	0.6 mM
13	GGTGTGC	natural abundance	0.6 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	D2O	[U-100% 2H]	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	water proton	4.802 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	water proton	4.802 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	water proton	4.802 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	water proton	4.802 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 8 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 4CH1, Strand ID: A Detail

Release date

2014-02-10

Citation 1

Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans
Amrane, S., Mackereth, C.D.
Biomol. NMR Assign. (2014), 8, 109-112, PubMed 23334698 , DOI 10.1007/s12104-013-9463-9 , Abstract

Show more 1 citaion

Citation 2

Backbone-independent nucleic acid binding by splicing factor SUP-12 reveals key aspects of molecular recognition
Amrane, S., Rebora, K., Zniber, I., Dupuy, D., Mackereth, C.D.
Nat. Commun. (2014), 5, 4595-4595, PubMed 25183497 , DOI 10.1038/ncomms5595 , Abstract

Hide non-primary 1 citaion

Related entities 1. PROTEIN SUP-12 ISOFORM B, : 1 : 2 : 149 entities Detail

Experiments performed 7 experiments Detail

1 15N-NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.8mM

#	Name	Isotope labeling	Concentration
1	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.8 mM
2	GGTGTGC	natural abundance	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	10 %
6	D2O	[U-100% 2H]	90 %

2 13C-NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.4mM

#	Name	Isotope labeling	Concentration
7	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.4 mM
8	GGTGTGC	natural abundance	0.4 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	[U-100% 2H]	100 %

3 double-filtered 1H

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.4mM

#	Name	Isotope labeling	Concentration
7	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.4 mM
8	GGTGTGC	natural abundance	0.4 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	[U-100% 2H]	100 %

4 1H-NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.4mM

#	Name	Isotope labeling	Concentration
7	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.4 mM
8	GGTGTGC	natural abundance	0.4 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	[U-100% 2H]	100 %

5 1H-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.4mM

#	Name	Isotope labeling	Concentration
7	PROTEIN SUP-12, ISOFORM B	[U-13C; U-15N]	0.4 mM
8	GGTGTGC	natural abundance	0.4 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	[U-100% 2H]	100 %

6 1H

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.6mM

#	Name	Isotope labeling	Concentration
12	PROTEIN SUP-12, ISOFORM B	[U-100% 2H]	0.6 mM
13	GGTGTGC	natural abundance	0.6 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	D2O	[U-100% 2H]	100 %

7 1H-NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.6mM

#	Name	Isotope labeling	Concentration
12	PROTEIN SUP-12, ISOFORM B	[U-100% 2H]	0.6 mM
13	GGTGTGC	natural abundance	0.6 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	D2O	[U-100% 2H]	100 %

nullKeywords alternative splicing, development, muscle, TRANSCRIPTION-DNA COMPLEX

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Found 1 Document (0.849 seconds)

BMRB: 19653

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PROTEIN SUP-12 ISOFORM B, : 1 : 2 : 149 entities Detail

Experiments performed 7 experiments Detail

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