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Found 1 Document (1.213 seconds)

BMRB: 19872

2MMZ

Solution structure of the apo form of human glutaredoxin 5

Authors

Banci, L., Brancaccio, D., Ciofi-Baffoni, S., Del Conte, R., Gadepalli, R., Mikolajczyk, M., Neri, S., Piccioli, M., Winkelmann, J.

Assembly

apo form of human glutaredoxin 5

Entity

1. apo form of human glutaredoxin 5 (polymer, Thiol state: all free), 121 monomers, 13157.78 Da Detail

GSFTMGAGGG GSAEQLDALV KKDKVVVFLK GTPEQPQCGF SNAVVQILRL HGVRDYAAYN VLDDPELRQG IKDYSNWPTI PQVYLNGEFV GGCDILLQMH QNGDLVEELK KLGIHSALLD E

Formula weight

13157.78 Da

Source organism

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 82.3 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (1137 of 1381)	75.4 % (539 of 715)	90.3 % (484 of 536)	87.7 % (114 of 130)
Backbone	82.7 % (592 of 716)	53.6 % (135 of 252)	98.6 % (343 of 348)	98.3 % (114 of 116)
Sidechain	84.4 % (651 of 771)	87.3 % (404 of 463)	84.0 % (247 of 294)	0.0 % (0 of 14)
Aromatic	29.2 % (28 of 96)	58.3 % (28 of 48)	0.0 % (0 of 47)	0.0 % (0 of 1)
Methyl	97.9 % (141 of 144)	95.8 % (69 of 72)	100.0 % (72 of 72)

1. entity

GSFTMGAGGG GSAEQLDALV KKDKVVVFLK GTPEQPQCGF SNAVVQILRL HGVRDYAAYN VLDDPELRQG IKDYSNWPTI PQVYLNGEFV GGCDILLQMH QNGDLVEELK KLGIHSALLD E

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	apo glutaredoxin 5	[U-100% 15N]		0.5 ~ 1.0 mM
2	potassium phosphate	natural abundance		50 mM
3	DTT	natural abundance		5 mM
4	GSH	natural abundance		5 mM
5	D2O	natural abundance		90 %
6	H2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.53 ppm, Outliers: 4 Detail

LACS Plot; CB

Referencing offset: 0.53 ppm, Outliers: 4 Detail

LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MMZ, Strand ID: A Detail

Release date

2015-06-04

Citation

[2Fe-2S] cluster transfer in iron-sulfur protein biogenesis
Banci, L., Brancaccio, D., Ciofi-Baffoni, S., Del Conte, R., Gadepalli, R., Mikolajczyk, M., Neri, S., Piccioli, M., Winkelmann, J.
Proc. Natl. Acad. Sci. U. S. A. (2014), 111, 6203-6208, PubMed 24733926 , DOI 10.1073/pnas.1400102111 , Abstract

Related entities 1. apo form of human glutaredoxin 5, : 1 : 2 : 162 entities Detail

Interaction partners 1. apo form of human glutaredoxin 5, : 7 interactors Detail

More details for 7 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	apo glutaredoxin 5	[U-100% 15N]		0.5 ~ 1.0 mM
2	potassium phosphate	natural abundance		50 mM
3	DTT	natural abundance		5 mM
4	GSH	natural abundance		5 mM
5	D2O	natural abundance		90 %
6	H2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

3 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

4 2D 1H-1H NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	apo glutaredoxin 5	[U-100% 15N]		0.5 ~ 1.0 mM
2	potassium phosphate	natural abundance		50 mM
3	DTT	natural abundance		5 mM
4	GSH	natural abundance		5 mM
5	D2O	natural abundance		90 %
6	H2O	natural abundance		10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

6 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

7 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

8 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

10 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	apo glutaredoxin 5	[U-100% 15N]		0.5 ~ 1.0 mM
2	potassium phosphate	natural abundance		50 mM
3	DTT	natural abundance		5 mM
4	GSH	natural abundance		5 mM
5	D2O	natural abundance		90 %
6	H2O	natural abundance		10 %

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

13 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
7	apo glutaredoxin 5	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
8	potassium phosphate	natural abundance		50 mM
9	DTT	natural abundance		5 mM
10	GSH	natural abundance		5 mM
11	D2O	natural abundance		90 %
12	H2O	natural abundance		10 %

nullKeywords [2Fe-2S] cluster protein, iron-sulfur protein biogenesis, monothiol glutaredoxins