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REFINED STRUCTURE OF OUTER MEMBRANE PROTEIN X IN NANODISC BY MEASURING RESIDUAL DIPOLAR COUPLINGS
Authors
Bibow, S., Carneiro, M.G., Sabo, T.M., Schwiegk, C., Becker, S., Riek, R., Lee, D.
Assembly
OUTER MEMBRANE PROTEIN X IN NANODISC
Entity
1. OUTER MEMBRANE PROTEIN X IN NANODISC (polymer, Thiol state: not present), 148 monomers, 16359.65 Da Detail

ATSTVTGGYA QSDAQGQMNK MGGFNLKYRY EEDNSPLGVI GSFTYTEKSR TASSGDYNKN QYYGITAGPA YRINDWASIY GVVGVGYGKF QTTEYPTYKN DTSDYGFSYG AGLQFNPMEN VALDFSYEQS RIRSVDVGTW IAGVGYRF


Formula weight
16359.65 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 80.4 %, Completeness: 23.5 %, Completeness (bb): 50.2 % Detail

Polymer type: polypeptide(L)

Total1H15N
All23.5 % (241 of 1025)14.4 % (123 of 857)70.2 % (118 of 168)
Backbone50.2 % (219 of 436)34.6 % (101 of 292)81.9 % (118 of 144)
Sidechain 3.7 % (22 of 589) 3.9 % (22 of 565) 0.0 % (0 of 24)
Aromatic 0.0 % (0 of 113) 0.0 % (0 of 111) 0.0 % (0 of 2)
Methyl 3.3 % (2 of 60) 3.3 % (2 of 60)

1. OUTER MEMBRANE PROTEIN X

ATSTVTGGYA QSDAQGQMNK MGGFNLKYRY EEDNSPLGVI GSFTYTEKSR TASSGDYNKN QYYGITAGPA YRINDWASIY GVVGVGYGKF QTTEYPTYKN DTSDYGFSYG AGLQFNPMEN VALDFSYEQS RIRSVDVGTW IAGVGYRF

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 7.4, Details 10 MM TRIS-HCL, 100 MM NACL, 1 MM EDTA, 0.05 % SODIUM AZIDE, 0.5 MM


#NameIsotope labelingTypeConcentration
1OUTER MEMBRANE PROTEIN X[U-100% 13C; U-100% 15N]0.5 mM
2Tris-HClnatural abundance10 mM
3sodium azidenatural abundance0.05 %
4NaClnatural abundance100 mM
5EDTAnatural abundance1 mM
6H2Onatural abundance90 %
7D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 10 models in PDB: 2MNH, Strand ID: A Detail


Release date
2014-04-13
Citation
Measuring membrane protein bond orientations in nanodiscs via residual dipolar couplings
Bibow, S., Carneiro, M.G., Sabo, T.M., Schwiegk, C., Becker, S., Riek, R., Lee, D.
Protein Sci. (2014), 23, 851-856, PubMed 24752984 , DOI 10.1002/pro.2482 ,
Related entities 1. OUTER MEMBRANE PROTEIN X IN NANODISC, : 1 : 5 : 3 : 10 entities Detail
Experiments performed 4 experiments Detail
Keywords BETA BARREL, MEMBRANE PROTEIN, NANODISC, OMPX, RESIDUAL DIPOLAR COUPLING