BMRB: 19958

Protein Phosphorylation upon a Fleeting Encounter

Authors

Xing, Q.S., Yang, J., Huang, P., Zhang, W., Tang, C.M.

Assembly

N-terminal Domain of Enzyme I

Entity

1. N-terminal Domain of Enzyme I (polymer, Thiol state: not present), 259 monomers, 28345.97 Da Detail

MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDR

2. EIN EIIAGlc (polymer, Thiol state: not present), 168 monomers, 18119.63 Da Detail

GLFDKLKSLV SDDKKDTGTI EIIAPLSGEI VNIEDVPDVV FAEKIVGDGI AIKPTGNKMV APVDGTIGKI FETNHAFSIE SDSGVELFVH FGIDTVELKG EGFKRIAEEG QRVKVGDTVI EFDLPLLEEK AKSTLTPVVI SNMDEIKELI KLSGSVTVGE TPVIRIKK

3. PHOSPHITE ION (non-polymer), 78.972 Da

Total weight

46544.574 Da

Max. entity weight

28345.97 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 67.0 %, Completeness: 18.8 %, Completeness (bb): 36.1 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	15N
All	18.8 % (558 of 2962)	10.9 % (274 of 2523)	64.7 % (284 of 439)
Backbone	36.1 % (466 of 1290)	20.9 % (183 of 874)	68.0 % (283 of 416)
Sidechain	5.6 % (93 of 1672)	5.6 % (93 of 1649)	0.0 % (0 of 23)
Aromatic	5.6 % (5 of 89)	5.6 % (5 of 89)
Methyl	7.0 % (21 of 300)	7.0 % (21 of 300)

1. N-terminal Domain of Enzyme I

MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDR

2. EIN EIIAGlc

GLFDKLKSLV SDDKKDTGTI EIIAPLSGEI VNIEDVPDVV FAEKIVGDGI AIKPTGNKMV APVDGTIGKI FETNHAFSIE SDSGVELFVH FGIDTVELKG EGFKRIAEEG QRVKVGDTVI EFDLPLLEEK AKSTLTPVVI SNMDEIKELI KLSGSVTVGE TPVIRIKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 313.4 (±2) K, pH 7.4 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal Domain of Enzyme I	[U-99% 15N]		0.5 mM
2	Enzyme II of Glucose	[U-99% 15N]		0.5 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 1 models in PDB: 2MP0, Strand ID: A, B Detail

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Release date

2014-05-21

Citation 1

Protein Phosphorylation upon a Fleeting Encounter
Xing, Q.S., Ju, Y., Huang, P., Zhang, W., Tang, C.M.
Nature

Show more 1 citaion

Citation 2

Protein Phosphorylation upon a Fleeting Encounter
Xing, Q.S., Ju, Y.G., Huang, P., Zhang, W., Tang, C.J.
Nature

Hide non-primary 1 citaion

Related entities 1. N-terminal Domain of Enzyme I, : 1 : 3 entities Detail

Related entities 2. EIN EIIAGlc, : 1 : 11 : 2 : 40 entities Detail

Interaction partners 2. EIN EIIAGlc, : 1 interactors Detail

Experiments performed 2 experiments Detail