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BMRB: 19974

2MPB

NMR structure of BA42 protein from the psychrophilic bacteria Bizionia argentinensis sp. nov.

Authors

Cicero, D., Aran, M., Smal, C., Pellizza, L., Gallo, M.

Assembly

BA42 protein

Entity

1. BA42 protein (polymer, Thiol state: not present), 145 monomers, 16477.40 Da Detail

MSKIEEFLTA EEEKAIVDAI RDAEKNTSGE IRVHLEKTSE IDVFDRAMDV FHNLKMDNTK LQNGVLIYVA VEDKTFVIYG DKGINDVVSD DFWDTTRNAI QLQFKQGNFK QGLVDGIEKA GMALAKYFPW KKDDIDELPN TISKG

Formula weight

16477.4 Da

Source organism

Bizionia argentinensis JUB59

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 92.9 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (1595 of 1716)	91.1 % (812 of 891)	95.7 % (638 of 667)	91.8 % (145 of 158)
Backbone	98.3 % (851 of 866)	97.3 % (289 of 297)	98.6 % (420 of 426)	99.3 % (142 of 143)
Sidechain	89.1 % (879 of 986)	88.0 % (523 of 594)	93.6 % (353 of 377)	20.0 % (3 of 15)
Aromatic	79.4 % (108 of 136)	85.3 % (58 of 68)	72.7 % (48 of 66)	100.0 % (2 of 2)
Methyl	98.8 % (162 of 164)	97.6 % (80 of 82)	100.0 % (82 of 82)

1. entity

MSKIEEFLTA EEEKAIVDAI RDAEKNTSGE IRVHLEKTSE IDVFDRAMDV FHNLKMDNTK LQNGVLIYVA VEDKTFVIYG DKGINDVVSD DFWDTTRNAI QLQFKQGNFK QGLVDGIEKA GMALAKYFPW KKDDIDELPN TISKG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 301 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	10 mM
2	D2O	[U-100% 2H]	5 %
3	H2O	natural abundance	95 %
4	entity	[U-15N; U-13C]	0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MPB, Strand ID: A Detail

Release date

2014-05-18

Citation

Solution and crystal structure of BA42, a protein from the Antarctic bacterium Bizionia argentinensis comprised of a stand-alone TPM domain
Aran, M., Smal, C., Pellizza, L., Gallo, M., Otero, L.H., Klinke, S., Goldbaum, F.A., Ithurralde, E.R., Bercovich, A., MacCormack, W.P., Turjanski, A.G., Cicero, D.O.
Proteins (2014), 82, 3062-3078, PubMed 25116514 , DOI 10.1002/prot.24667 , Abstract

Related entities 1. BA42 protein, : 1 : 2 : 3 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC