NMR Solution Structure of Leucine-Enkephlain in DMPC+CHAPS (1:4) bicelles in 90% H2O/10% D2O of pH 6.6 at 298K
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 81.8 % (45 of 55) | 80.0 % (24 of 30) | 84.0 % (21 of 25) |
Backbone | 96.0 % (24 of 25) | 91.7 % (11 of 12) | 100.0 % (13 of 13) |
Sidechain | 72.7 % (24 of 33) | 72.2 % (13 of 18) | 73.3 % (11 of 15) |
Aromatic | 55.6 % (10 of 18) | 55.6 % (5 of 9) | 55.6 % (5 of 9) |
Methyl | 100.0 % (4 of 4) | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. Leu-Enkephalin
YGGFLSolvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.6, Details Lyophilized powder from Sigma-Aldrich dissolved in isotropic DMPC+CHAPS+GM1 (1:4:0.3) bicelles in 90%H2O/10% D2O (pH 6.6 at 298 K) with peptide:lipid 1:13
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Leu-Enkephalin | natural abundance | 4-5 mM | |
2 | DMPC+CHAPS+GM1 | natural abundance | 4 % w/v |