BMRBj - BMREntryMaster

Found 1 Document (1.031 seconds)

BMRB: 20047

3J35

INTERACTION OF THE DENGUE VIRUS FUSION PEPTIDE WITH MEMBRANES ASSESSED BY NMR: THE ESSENTIAL ROLE OF THE ENVELOPE PROTEIN TRP101 FOR MEMBRANE FUSION

Authors

Melo, M.N., Sousa, F.J.R., Carneiro, F.A., Castanho, M.A.R.B., Valente, A., Almeida, F.C.L., da Poian, A.T., Mohana-Borges, R.

Assembly

fusionpeptide

Entity

1. fusionpeptide (polymer, Thiol state: all free), 15 monomers, 1480.609 Da Detail

DRGWGNGCGL FGKGG

Formula weight

1480.609 Da

Source organism

Dengue virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 81.3 %, Completeness (bb): 89.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	81.3 % (65 of 80)	81.3 % (65 of 80)
Backbone	89.2 % (33 of 37)	89.2 % (33 of 37)
Sidechain	74.4 % (32 of 43)	74.4 % (32 of 43)
Aromatic	81.8 % (9 of 11)	81.8 % (9 of 11)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. fusionpeptide

DRGWGNGCGL FGKGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 278 K, pH 5.5, Details dodecylphosphocholine (DPC) micelles

#	Name	Isotope labeling	Concentration
1	fusionpeptide	natural abundance	2 mM
2	DPC	[U-99% 2H]	100 mM
3	DTT	[U-99% 2H]	10 mM
4	sodium phosphate	natural abundance	100 mM
5	sodium azide	natural abundance	0.01 %
6	D2O	[U-99% 2H]	10 %

Release date

2009-07-22

Citation

Interaction of the Dengue virus fusion peptide with membranes assessed by NMR: The essential role of the envelope protein Trp101 for membrane fusion
Melo, M.N., Sousa, F.J.R., Carneiro, F.A., Castanho, M.A.R.B., Valente, A., Almeida, F.C.L., da Poian, A.T., Mohana-Borges, R.
J. Mol. Biol. (2009), 392, 736-746, PubMed 19619560 , DOI 10.1016/j.jmb.2009.07.035 , Abstract

Related entities 1. fusionpeptide, : 1 : 63 : 93 : 1 entities Detail

Interaction partners 1. fusionpeptide, : 58 interactors Detail

More details for 58 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr20047_3.str
Input source #2: Coordindates	bmr20047.coor.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): 1 vs Auth_asym_ID (model): A

--------10-----
DRGWGNGCGLFGKGG
|||||||||||||||
DRGWGNGCGLFGKGG

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
1	A	15	0	0	100.0

Content subtype: bmr20047_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	71		93.3 (chain: 1, length: 15)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 15, Sequence coverage: 93.3%
- Total number of assignments
  - ¹H chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
2	ARG	HH12	6.728
2	ARG	HH21	7.098

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	65	81.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	37	33	89.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	32	74.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	9	81.8

Keywords dengue, DPC, Fusion Peptide

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Found 1 Document (1.031 seconds)

BMRB: 20047

Sample

Related entities 1. fusionpeptide, : 1 : 63 : 93 : 1 entities Detail

Interaction partners 1. fusionpeptide, : 58 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail