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Reexamination of the Secondary and Tertiary Structure of Histidine-Containing Protein from Escherichia coli by Homonuclear and Heteronuclear NMR Spectroscopy
Authors
Hammen, P.K., Waygood, E.BRUCE., Klevit, R.E.
Assembly
phosphocarrier protein HPr
Entity
1. phosphocarrier protein HPr (polymer), 85 monomers, 9119.249 Da Detail

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE


Formula weight
9119.249 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

Total1H15N
All93.0 % (545 of 586)93.5 % (462 of 494)90.2 % (83 of 92)
Backbone100.0 % (251 of 251)100.0 % (168 of 168)100.0 % (83 of 83)
Sidechain87.8 % (294 of 335)90.2 % (294 of 326) 0.0 % (0 of 9)
Aromatic100.0 % (24 of 24)100.0 % (24 of 24)
Methyl89.1 % (49 of 55)89.1 % (49 of 55)

1. phosphocarrier protein HPr

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Sample

Temperature 303 K, pH 6.5



Release date
1995-07-30
Citation
Reexamination of the secondary and tertiary structure of histidine-containing protein from Escherichia coli by homonuclear and heteronuclear NMR spectroscopy
Hammen, P.K., Waygood, E.BRUCE., Klevit, R.E.
Biochemistry (1991), 30, 11842-11850, PubMed 1751501 , DOI 10.1021/bi00115a014 ,
Related entities 1. phosphocarrier protein HPr, : 1 : 15 : 1 : 4 : 89 entities Detail
Interaction partners 1. phosphocarrier protein HPr, : 10 interactors Detail
Experiments performed 1 experiments Detail