BMRBj - BMREntryMaster

Found 1 Document (1.127 seconds)

BMRB: 2066

1BNR

Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR

Authors

Meiering, E.M., Bycroft, M., Fersht, A.R.

Assembly

barnase

Entity

1. barnase (polymer), 110 monomers, 12382.59 Da Detail

AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR

Formula weight

12382.59 Da

Source organism

Bacillus amyloliquefaciens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 33.6 %, Completeness: 13.0 %, Completeness (bb): 21.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	13.0 % (86 of 662)	13.0 % (86 of 662)
Backbone	21.1 % (48 of 227)	21.1 % (48 of 227)
Sidechain	8.7 % (38 of 435)	8.7 % (38 of 435)
Aromatic	14.3 % (10 of 70)	14.3 % (10 of 70)
Methyl	7.3 % (4 of 55)	7.3 % (4 of 55)

1. barnase

AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR

Show sample condition

Sample

Temperature 310 K, pH 4.5

Release date

1995-07-30

Citation

Characterization of phosphate binding in the active site of barnase by site-directed mutagenesis and NMR
Meiering, E.M., Bycroft, M., Fersht, A.R.
Biochemistry (1991), 30, 11348-11356, PubMed 1958671 , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 2067 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2068 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2069 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2070 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2071 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr2066_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.127 seconds)

BMRB: 2066

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. barnase, : 1 : 15 : 9 : 26 : 12 entities Detail

Interaction partners 1. barnase, : 1 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail