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Structural characterization of a 39-residue synthetic peptide containing the two zinc binding domains from the HIV-1 p7 nucleocapsid protein by CD and NMR spectroscopy
Authors
Omichinski, J.G., Clore, G.MARIUS., Sakaguchi, K., Appella, E., Gronenborn, A.M.
Assembly
nucleocapsid protein
Entity
1. nucleocapsid protein (polymer), 39 monomers, 4426.118 Da Detail

VKCFNCGKEG HTARNCRAPR KKGCWKCGKE GHQMKDCTE


Formula weight
4426.118 Da
Source organism
Human immunodeficiency virus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 99.6 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

Total1H
All99.6 % (241 of 242)99.6 % (241 of 242)
Backbone98.8 % (81 of 82)98.8 % (81 of 82)
Sidechain100.0 % (160 of 160)100.0 % (160 of 160)
Aromatic100.0 % (15 of 15)100.0 % (15 of 15)
Methyl100.0 % (6 of 6)100.0 % (6 of 6)

1. nucleocapsid protein

VKCFNCGKEG HTARNCRAPR KKGCWKCGKE GHQMKDCTE

Sample

Temperature 288 K, pH 5.8



Protein Blocks Logo
Calculated from 1 models in PDB: 1NCP, Strand ID: C, N Detail


Release date
1995-07-30
Citation
Structural characterization of a 39-residue synthetic peptide containing the two zinc binding domains from the HIV-1 p7 nucleocapsid protein by CD and NMR spectroscopy
Omichinski, J.G., Clore, G.MARIUS., Sakaguchi, K., Appella, E., Gronenborn, A.M.
FEBS Lett. (1991), 292, 25-30, PubMed 1959614 ,
Related entities 1. nucleocapsid protein, : 1 : 9 : 2 : 10 : 300 entities Detail
Interaction partners 1. nucleocapsid protein, : 5 interactors Detail
Experiments performed 1 experiments Detail
Chemical shift validation 3 contents Detail