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Preparation of a Lysozyme Derivative in Which Two Domains Are Cross-Linked Intramolecularly between Trp62 and Asp101
Authors
Ueda, T., Yamada, H., Sakamoto, N., Abe, Y., Kawano, K., Terada, Y., Imoto, T.
Assembly
lysozyme
Entity
1. lysozyme (polymer), 129 monomers, 14297.99 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCALKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL


Formula weight
14297.99 Da
Source organism
Gallus gallus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 12.4 %, Completeness: 7.9 %, Completeness (bb): 3.0 % Detail

Polymer type: polypeptide(L)

Total1H
All 7.9 % (59 of 749) 7.9 % (59 of 749)
Backbone 3.0 % (8 of 268) 3.0 % (8 of 268)
Sidechain10.6 % (51 of 481)10.6 % (51 of 481)
Aromatic76.9 % (50 of 65)76.9 % (50 of 65)
Methyl 0.0 % (0 of 61) 0.0 % (0 of 61)

1. lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCALKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Sample

Temperature 308 K, pH 3.8



Release date
1995-07-30
Citation
Preparation and properties of a lysozyme derivative in which two domains are cross-linked intramolecularly between Trp62 and Asp101
Ueda, T., Yamada, H., Sakamoto, N., Abe, Y., Kawano, K., Terada, Y., Imoto, T.
J. Biochem. (1991), 110, 719-725, PubMed 1783601 ,
Related entities 1. lysozyme, : 1 : 105 : 142 entities Detail
Interaction partners 1. lysozyme, : 7 interactors Detail
Experiments performed 1 experiments Detail
Chemical shift validation 3 contents Detail