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Solution structure of the LysM region of the E. coli Intimin periplasmic domain
Authors
Coles, M., Chaubey, M., Leo, J.C., Linke, D., Schuetz, M.C., Goetz, F., Autenrieth, I.B.
Assembly
LysM region of the E. coli Intimin periplasmic domain
Entity
1. LysM region of the E. coli Intimin periplasmic domain (polymer, Thiol state: not present), 115 monomers, 12556.06 Da Detail

MNGENYFKLG SDSKLLTHNS YQNRLFYTLK TGETVADLSK SQDINLSTIW SLNKHLYSSE SEMMKAAPGQ QIILPLKKLP FEYSALPLLG SAPLVAAGGV AGHTNGSGSE NLYFQ


Formula weight
12556.06 Da
Source organism
Escherichia coli
Exptl. method
SOLUTION NMR
Refine. method
simulated annealing, NOESY back-calculation
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 76.5 %, Completeness: 65.0 %, Completeness (bb): 69.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All65.0 % (866 of 1332)66.3 % (457 of 689)62.5 % (324 of 518)68.0 % (85 of 125)
Backbone69.6 % (466 of 670)70.7 % (159 of 225)67.5 % (226 of 335)73.6 % (81 of 110)
Sidechain62.3 % (478 of 767)64.2 % (298 of 464)61.1 % (176 of 288)26.7 % (4 of 15)
Aromatic19.6 % (22 of 112)37.5 % (21 of 56) 0.0 % (0 of 55)100.0 % (1 of 1)
Methyl77.4 % (96 of 124)74.2 % (46 of 62)80.6 % (50 of 62)

1. LysM

MNGENYFKLG SDSKLLTHNS YQNRLFYTLK TGETVADLSK SQDINLSTIW SLNKHLYSSE SEMMKAAPGQ QIILPLKKLP FEYSALPLLG SAPLVAAGGV AGHTNGSGSE NLYFQ

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
6LysM[U-100% 13C; U-100% 15N]0.5 mM
7sodium phosphatenatural abundance20 mM
8sodium chloridenatural abundance150 mM
9H2Onatural abundance90 %
10D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: 0.12 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.12 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.1 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.09 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 23 models in PDB: 2MPW, Strand ID: A Detail


Release date
2014-11-09
Citation
The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan
Leo, J.C., Oberhettinger, P., Chaubey, M., Schuetz, M., Kuehner, D., Goetz, F., Coles, M., Autenrieth, I.B., Linke, D.
Mol. Microbiol. (2015), 95, 80-100, PubMed 25353290 , DOI 10.1111/mmi.12840 ,
Related entities 1. LysM region of the E. coli Intimin periplasmic domain, : 1 : 1 : 6 entities Detail
Experiments performed 12 experiments Detail
Keywords LysM, peptidoglycan-binding