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BMRB: 25225

6ZZE

Structure of the trans-(Tyr39-Pro40) form of the Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1)

Authors

Paramonov, A.S., Shenkarev, Z.O., Lyukmanova, E.N., Arseniev, A.S.

Assembly

trans-(Tyr39-Pro40) form of the Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1)

Entity

1. trans-(Tyr39-Pro40) form of the Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1) (polymer), 82 monomers, 8984.293 Da Detail

MLKCYTCKEP MTSASCRTIT RCKPEDTACM TTLVTVEAEY PFNQSPVVTR SCSSSCVATD PDSIGAAHLI FCCFRDLCNS EL

Formula weight

8984.293 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.8 %, Completeness (bb): 81.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.8 % (744 of 888)	93.0 % (423 of 455)	69.1 % (244 of 353)	96.2 % (77 of 80)
Backbone	81.1 % (391 of 482)	95.6 % (153 of 160)	66.1 % (162 of 245)	98.7 % (76 of 77)
Sidechain	88.5 % (431 of 487)	91.9 % (271 of 295)	84.1 % (159 of 189)	33.3 % (1 of 3)
Aromatic	32.0 % (16 of 50)	56.0 % (14 of 25)	8.0 % (2 of 25)
Methyl	95.2 % (80 of 84)	97.6 % (41 of 42)	92.9 % (39 of 42)

1. Slurp1

MLKCYTCKEP MTSASCRTIT RCKPEDTACM TTLVTVEAEY PFNQSPVVTR SCSSSCVATD PDSIGAAHLI FCCFRDLCNS EL

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 4.7

#	Name	Isotope labeling	Concentration
1	Slurp1	[U-100% 15N]	0.3 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 4.7

#	Name	Isotope labeling	Concentration
4	Slurp1	[U-100% 13C; U-100% 15N]	0.3 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 4.7

#	Name	Isotope labeling	Type	Concentration
7	Slurp1	[U-100% 13C; U-100% 15N]		0.3 mM
8	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.98 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.98 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MUO, Strand ID: A Detail

Release date

2015-12-06

Citation

Structural and Functional Properties of Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1) Imply a Non-Canonical Mode of Interaction with 7 nAChR
Lyukmanova, E.N., Shenkarev, Z.O., Shulepko, M.A., Paramonov, A.S., Kudryavsev, D.S., Astapova, M.V., Tompsen, M.S., Kasheverov, I.E., Feofanov, A.V., Arseniev, A.S., Tsetlin, V.I., Dolgikh, D.A., Kirpichnikov, M.P.
FEBS Lett.

Related entities 1. trans-(Tyr39-Pro40) form of the Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1), : 1 : 1 : 2 : 49 entities Detail

Interaction partners 1. trans-(Tyr39-Pro40) form of the Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1), : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC