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BMRB: 25235

2MUY

The solution structure of the FtsH periplasmic N-domain

Authors

Scharfenberg, F., Serek-Heuberger, J., Martin, J., Lupas, A.N., Coles, M.

Assembly

FtsH periplasmic N-domain

Entity

1. FtsH periplasmic N-domain (polymer, Thiol state: not present), 82 monomers, 9528.433 Da Detail

MASESNGRKV DYSTFLQEVN NDQVREARIN GREINVTKKD SNRYTTYIPV QDPKLLDNLL TKNVKVVGEP PEEPLEHHHH HH

Formula weight

9528.433 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing, NOESY back-calculation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 4.9 %, Completeness: 4.1 %, Completeness (bb): 4.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	4.1 % (40 of 969)	4.5 % (23 of 510)	3.5 % (13 of 371)	4.5 % (4 of 88)
Backbone	4.1 % (20 of 482)	4.3 % (7 of 162)	4.1 % (10 of 243)	3.9 % (3 of 77)
Sidechain	4.1 % (23 of 566)	4.6 % (16 of 348)	2.9 % (6 of 207)	9.1 % (1 of 11)
Aromatic	0.0 % (0 of 58)	0.0 % (0 of 29)	0.0 % (0 of 29)
Methyl	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 41)

1. FtsH-N

MASESNGRKV DYSTFLQEVN NDQVREARIN GREINVTKKD SNRYTTYIPV QDPKLLDNLL TKNVKVVGEP PEEPLEHHHH HH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	FtsH-N	[U-100% 15N]		0.7 mM
2	sodium phosphate	natural abundance		15 mM
3	sodium chloride	natural abundance		75 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2MUY, Strand ID: A Detail

Release date

2016-09-21

Citation

Structure and evolution of N-domains in AAA metalloproteases
Scharfenberg, F., Serek-Heuberger, J., Coles, M., Hartmann, M.D., Habeck, M., Martin, J., Lupas, A.N., Alva, V.
J. Mol. Biol. (2015), 427, 910-923, PubMed 25576874 , DOI 10.1016/j.jmb.2014.12.024 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25241 released on 2016-09-21
Title The N-domain of the AAA metalloproteinase Yme1 from Saccharomyces cerevisiae

Related entities 1. FtsH periplasmic N-domain, : 1 : 1 : 6 : 16 entities Detail

Interaction partners 1. FtsH periplasmic N-domain, : 29 interactors Detail

More details for 29 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

2 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

3 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

4 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

6 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

7 3D CCH NOESY

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

8 3D CNH NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	FtsH-N	[U-100% 15N]		0.7 mM
2	sodium phosphate	natural abundance		15 mM
3	sodium chloride	natural abundance		75 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
4	FtsH-N	[U-100% 13C; U-100% 15N]		0.7 mM
5	sodium phosphate	natural abundance		15 mM
6	sodium chloride	natural abundance		75 mM

11 3D HNHA

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	FtsH-N	[U-100% 15N]		0.7 mM
2	sodium phosphate	natural abundance		15 mM
3	sodium chloride	natural abundance		75 mM

12 3D HNHB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	FtsH-N	[U-100% 15N]		0.7 mM
2	sodium phosphate	natural abundance		15 mM
3	sodium chloride	natural abundance		75 mM

nullKeywords AAA ATPase, metalloprotease, substrate recognition domain