Solution structure of [GlnB22]-insulin mutant at pH 1.9
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS6:SG | 1:CYS11:SG |
2 | disulfide | sing | 1:CYS7:SG | 2:CYS7:SG |
3 | disulfide | sing | 1:CYS20:SG | 2:CYS19:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 95.7 % (288 of 301) | 95.7 % (288 of 301) |
Backbone | 95.2 % (100 of 105) | 95.2 % (100 of 105) |
Sidechain | 95.9 % (188 of 196) | 95.9 % (188 of 196) |
Aromatic | 97.1 % (34 of 35) | 97.1 % (34 of 35) |
Methyl | 100.0 % (28 of 28) | 100.0 % (28 of 28) |
1. entity 1
GIVEQCCTSI CSLYQLENYC N2. entity 2
FVNQHLCGSH LVEALYLVCG EQGFFYTPKTSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 1.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | natural abundance | 0.2 mM | |
2 | entity_2 | natural abundance | 0.2 mM | |
3 | acetic acid | [U-99% 2H] | 20 % | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | [U-99% 2H] | 5 % |