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BMRB: 25650

2N3L

Solution structure of RNA recognition motif-1 of Plasmodium falciparum serine/arginine-rich protein 1.

Authors

Ganguly, A., Verma, G., Bhavesh, N.

Assembly

RNA recognition motif-1 of serine/arginine-rich protein 1

Entity

1. RNA recognition motif-1 of serine/arginine-rich protein 1 (polymer), 89 monomers, 9981.065 Da Detail

GSHMVIRESV SRIYVGNLPS HVSSRDVENE FRKYGNILKC DVKKTVSGAA FAFIEFEDAR DAADAIKEKD GCDFEGNKLR VEVPFNARE

Formula weight

9981.065 Da

Source organism

Plasmodium falciparum

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 85.6 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.6 % (872 of 1019)	80.8 % (429 of 531)	90.4 % (358 of 396)	92.4 % (85 of 92)
Backbone	96.6 % (512 of 530)	96.7 % (176 of 182)	96.6 % (252 of 261)	96.6 % (84 of 87)
Sidechain	76.0 % (435 of 572)	71.3 % (249 of 349)	85.3 % (186 of 218)	0.0 % (0 of 5)
Aromatic	42.9 % (36 of 84)	42.9 % (18 of 42)	42.9 % (18 of 42)
Methyl	91.9 % (79 of 86)	88.4 % (38 of 43)	95.3 % (41 of 43)

1. PfSR1-RRM1

GSHMVIRESV SRIYVGNLPS HVSSRDVENE FRKYGNILKC DVKKTVSGAA FAFIEFEDAR DAADAIKEKD GCDFEGNKLR VEVPFNARE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Concentration
1	PfSR1-RRM1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	3 mM
5	L-arginine	natural abundance	50 mM
6	L-glutamate	natural abundance	25 mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Concentration
9	PfSR1-RRM1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	TCEP	natural abundance	3 mM
13	L-arginine	natural abundance	50 mM
14	L-glutamate	natural abundance	25 mM
15	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N3L, Strand ID: A Detail

Release date

2016-07-17

Citation

The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition
Ganguly, A., Verma, G., Bhavesh, N.
J. Mol. Biol. (2018), PubMed 30500338 , DOI 10.1016/j.jmb.2018.11.020 , Abstract

Related entities 1. RNA recognition motif-1 of serine/arginine-rich protein 1, : 1 : 1 : 1 : 310 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC