BMRB: 25750

NMR Structure of the C-terminal Coiled-Coil Domain of CIN85

Authors

Habeck, M., Becker, S., Griesinger, C., Wong, L.E.

Assembly

CIN85 CC-domain trimer

Entity

1. CIN85 CC-domain trimer (polymer, Thiol state: not present), 75 monomers, 8745.047 × 3 Da Detail

GHMEGKPKME PAASSQAAVE ELRTQVRELR SIIETMKDQQ KREIKQLLSE LDEEKKIRLR LQMEVNDIKK ALQSK

Total weight

26235.14 Da

Max. entity weight

8745.047 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 65.3 %, Completeness (bb): 82.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	65.3 % (602 of 922)	47.9 % (238 of 497)	82.6 % (284 of 344)	98.8 % (80 of 81)
Backbone	82.1 % (366 of 446)	50.7 % (76 of 150)	97.8 % (218 of 223)	98.6 % (72 of 73)
Sidechain	56.1 % (308 of 549)	46.7 % (162 of 347)	71.1 % (138 of 194)	100.0 % (8 of 8)
Aromatic	0.0 % (0 of 4)	0.0 % (0 of 2)	0.0 % (0 of 2)
Methyl	93.6 % (73 of 78)	92.3 % (36 of 39)	94.9 % (37 of 39)

1. CIN85 CC-domain

GHMEGKPKME PAASSQAAVE ELRTQVRELR SIIETMKDQQ KREIKQLLSE LDEEKKIRLR LQMEVNDIKK ALQSK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	CIN85 CC-domain	[U-13C; U-15N; U-2H]		2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
2	CIN85 CC-domain	[U-13C; U-15N; U-2H]		2 mM
3	CIN85 CC-domain	[U-2H]		2 mM

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Protein Blocks Logo

Calculated from 30 models in PDB: 2N64, Strand ID: A, B, C Detail

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Heteronucl. T₂

60 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 800.05 MHz, Pressure 1 atm, Temperature 305 K, pH 6.9 Detail

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Pressure 1 atm, Temperature 305 K, pH 6.9

Experiment name 2D TRACT

Release date

2016-07-10

Citation

The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation
Kuehn, J., Wong, L.E., Pirkuliyeva, S., Batista, F.D., Schwiegk, C., Gencalp, K., Urlaub, H., Habeck, M., Becker, S., Griesinger, C., Wienands, J.
Sci. Signal. (2016), 9, ra66-ra66, PubMed 27353366 , DOI 10.1126/scisignal.aad6275 , Abstract

Related entities 1. CIN85 CC-domain trimer, : 2 : 19 entities Detail

Interaction partners 1. CIN85 CC-domain trimer, : 37 interactors Detail

Experiments performed 8 experiments Detail