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Found 1 Document (6.308 seconds)

BMRB: 25889

2N8A

1H, 13C and 15N chemical shift assignments for PARP-1 F1F2 domains (at 200mM NaCl)

Authors

Neuhaus, D., Eustermann, S., Yang, J., Wu, W.

Assembly

F1F2 free

Entity

1. F1F2 free (polymer), 214 monomers, 24062.18 Da Detail

MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD GIGSKAEKTL GDFAAEYAKS NRSTCKGCME KIEKGQVRLS KKMVDPEKPQ LGMIDRWYHP GCFVKNREEL GFRPEYSASQ LKGFSLLATE DKEALKKQLP GVKSEGKRKG DEVD

Formula weight

24062.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.7 %, Completeness: 26.6 %, Completeness (bb): 60.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	26.6 % (413 of 1550)	16.7 % (222 of 1331)	87.2 % (191 of 219)
Backbone	60.4 % (389 of 644)	45.1 % (198 of 439)	93.2 % (191 of 205)
Sidechain	2.8 % (25 of 906)	2.8 % (25 of 892)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 102)	0.0 % (0 of 98)	0.0 % (0 of 4)
Methyl	2.5 % (2 of 80)	2.5 % (2 of 80)

1. F1F2

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details Uniform [2H,15N,13C]

#	Name	Isotope labeling	Type	Concentration
1	PARP-1 1-214	[U-15N; U-13C; U-2H]		0.2 mM
2	TRIS	[U-2H]		50 mM
3	DTT	[U-2H]		1 mM
4	ZnSO4	natural abundance		0.1 uM
5	sodium chloride	natural abundance		200 mM
6	H2O	natural abundance	solvent	95 %
7	D2O	[U-2H]	solvent	5 %

Release date

2015-11-24

Citation

Structural Basis of Detection and Signaling of DNA Single-Strand Breaks by Human PARP-1
Eustermann, S., Wu, W., Langelier, M., Yang, J., Easton, L.E., Riccio, A., Pascal, J.M., Neuhaus, D.
Mol. Cell (2015), 60, 742-754, PubMed 26626479 , DOI 10.1016/j.molcel.2015.10.032 , Abstract

Entries sharing articles BMRB: 7 entries Detail

  BMRB: 25888 released on 2015-11-24
    Title 1H, 13C and 15N chemical shift assignments and solution structure for PARP-1 F1F2 domains in complex with a DNA single-strand break
  BMRB: 25890 released on 2015-11-24
    Title 1H chemical shift assignments for 45 nucleotide DNA dumbbell (model for single-strand break with one-nucleotide gap)
  BMRB: 25891 released on 2015-11-24
    Title 1H, 13C and 15N chemical shift assignments for PARP-1 F1F2F3 domains in complex with a DNA single-strand break
  BMRB: 25892 released on 2015-11-24
    Title 1H, 13C and 15N chemical shift assignments for PARP-1 F1F2F3 domains
  BMRB: 25893 released on 2015-11-24
    Title 1H, 13C and 15N chemical shift assignments for PARP-1 F3 domain
  BMRB: 25894 released on 2015-11-24
    Title 1H and 15N chemical shift assignments for PARP-1 F1F2F3 domains in complex with PARP-1 WGR domain and a DNA single-strand break
  BMRB: 25895 released on 2015-11-24
    Title 1H, 13C and 15N chemical shift assignments for PARP-1 WGR domain

Related entities 1. F1F2 free, : 1 : 2 : 23 entities Detail

Interaction partners 1. F1F2 free, : 182 interactors Detail

More details for 182 interactors identified by 72 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_free_F1F2_high_salt	Warning	382		89.3 (chain: 1, length: 214)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1331	191	14.4
¹⁵N chemical shifts	230	191	83.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	439	191	43.5
¹⁵N chemical shifts	205	191	93.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	892	0	0.0
¹⁵N chemical shifts	25	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	0	0.0
¹⁵N chemical shifts	4	0	0.0

Keywords PROTEIN/DNA, Structural basis of detection and signaling of DNA single-strand breaks by human PARP 1

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr25889_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (6.308 seconds)

BMRB: 25889

Sample

Entries sharing articles BMRB: 7 entries Detail

Related entities 1. F1F2 free, : 1 : 2 : 23 entities Detail

Interaction partners 1. F1F2 free, : 182 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail