BMRB: 27227

Backbone 1H and 15N Chemical Shift Assignments for mSin3B

Authors

Kurita, J., Hirao, Y., Nishimura, Y.

Assembly

mSin3B PAH1

Entity

1. mSin3B PAH1 (polymer, Thiol state: not present), 84 monomers, 9584.896 Da Detail

GSHMEKLPVH VEDALTYLDQ VKIRFGSDPA TYNGFLEIMK EFKSQSIDTP GVIRRVSQLF HEHPDLIVGF NAFLPLGYRI DIPK

Formula weight

9584.896 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 57.1 %, Completeness: 18.2 %, Completeness (bb): 39.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	18.2 % (111 of 611)	12.1 % (64 of 528)	56.6 % (47 of 83)
Backbone	39.4 % (97 of 246)	29.8 % (50 of 168)	60.3 % (47 of 78)
Sidechain	3.8 % (14 of 365)	3.9 % (14 of 360)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 50)	0.0 % (0 of 50)
Methyl	4.2 % (2 of 48)	4.2 % (2 of 48)

1. mSin3B

GSHMEKLPVH VEDALTYLDQ VKIRFGSDPA TYNGFLEIMK EFKSQSIDTP GVIRRVSQLF HEHPDLIVGF NAFLPLGYRI DIPK

Show sample condition

Sample

Solvent system 95%H2O/5%DMSO, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	mSin3B	[U-98% 15N]		100 uM

Hide sample codition

Release date

2017-08-21

Citation

A mimetic of the mSin3-binding helix of NRSF/REST ameliorates abnormal pain behavior in chronic pain models
Ueda, H., Kurita, J.I., Neyama, H., Hirao, Y., Kouji, H., Mishina, T., Kasai, M., Nakano, H., Yoshimori, A., Nishimura, Y.
Bioorg. Med. Chem. Lett. (2017), 27, 4705-4709, PubMed 28927787 , DOI 10.1016/j.bmcl.2017.09.006 , Abstract

Related entities 1. mSin3B PAH1, : 1 : 4 : 186 entities Detail

Interaction partners 1. mSin3B PAH1, : 9 interactors Detail

Experiments performed 1 experiments Detail