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BMRB: 27243

6AX5

RPT1 region of INI1/SNF5/SMARCB1_HUMAN - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

Authors

Girvin, M.E., Cahill, S.M., Harris, R., Cowburn, D., Spira, M., Wu, X., Prakash, R., Brenowitz, M., Almo, S.C., Kalpana, G.V.

Assembly

Rpt1/ini1

Entity

1. Rpt1/ini1 (polymer, Thiol state: not present), 83 monomers, 9565.857 Da Detail

PEVLVPIRLD MEIDGQKLRD AFTWNMNEKL MTPEMFSEIL CDDLDLNPLT FVPAIASAIR QQIESYPTDS ILEDQSDQRV IIK

Formula weight

9565.857 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.4 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.4 % (975 of 991)	97.1 % (503 of 518)	99.7 % (386 of 387)	100.0 % (86 of 86)
Backbone	98.6 % (479 of 486)	96.3 % (155 of 161)	99.6 % (247 of 248)	100.0 % (77 of 77)
Sidechain	98.5 % (578 of 587)	97.5 % (348 of 357)	100.0 % (221 of 221)	100.0 % (9 of 9)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (24 of 24)	100.0 % (1 of 1)
Methyl	99.0 % (103 of 104)	98.1 % (51 of 52)	100.0 % (52 of 52)

1. Rpt1-ini1

PEVLVPIRLD MEIDGQKLRD AFTWNMNEKL MTPEMFSEIL CDDLDLNPLT FVPAIASAIR QQIESYPTDS ILEDQSDQRV IIK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Rpt1/ini1	[U-95% 13C; U-95% 15N]	250 uM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	EDTA	natural abundance	1 mM
5	TCEP	natural abundance	5 mM
6	TSP	natural abundance	0.01 (±0.01) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	protons	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	protons	internal	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6AX5, Strand ID: A Detail

Release date

2017-08-31

Citation

RPT1 region of INI1/SNF5/SMARCB1_HUMAN - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
Girvin, M.E., Cahill, S.M., Harris, R., Cowburn, D., Spira, M., Wu, X., Prakash, R., Brenowitz, M., Almo, S.C., Kalpana, G.V.
Not known

Related entities 1. Rpt1/ini1, : 4 : 21 entities Detail

Interaction partners 1. Rpt1/ini1, : 17 interactors Detail

More details for 17 interactors identified by 10 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC