BMRBj - BMREntryMaster

Found 1 Document (1.339 seconds)

BMRB: 2784

2SNS

Dynamics of Methyl Groups in Proteins As Studied by Proton-Detected 13C NMR Spectroscopy. Application to the Leucine Residues of Staphylococcal Nuclease

Authors

Nicholson, L.K., Kay, L.E., Baldisseri, D.M., Arango, J., Young, P.E., Bax, A., Torchia, D.A.

Assembly

micrococcal nuclease

Entity

1. micrococcal nuclease (polymer), 156 monomers, 17603.95 Da Detail

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Formula weight

17603.95 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 8.3 %, Completeness: 3.7 %, Completeness (bb): 1.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	3.7 % (63 of 1698)	3.6 % (35 of 980)	3.9 % (28 of 718)
Backbone	1.0 % (8 of 773)	0.6 % (2 of 315)	1.3 % (6 of 458)
Sidechain	5.3 % (57 of 1071)	5.0 % (33 of 665)	5.9 % (24 of 406)
Aromatic	0.0 % (0 of 121)	0.0 % (0 of 61)	0.0 % (0 of 60)
Methyl	29.2 % (45 of 154)	28.6 % (22 of 77)	29.9 % (23 of 77)

1. micrococcal nuclease

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Show sample condition

Sample

Temperature 310 K, pH 7

Release date

1995-07-30

Citation

Dynamics of methyl groups in proteins as studied by proton-detected 13C NMR spectroscopy. Application to the leucine residues of staphylococcal nuclease
Nicholson, L.K., Kay, L.E., Baldisseri, D.M., Arango, J., Young, P.E., Bax, A., Torchia, D.A.
Biochemistry (1992), 31, 5253-5263, PubMed 1606149 , DOI 10.1021/bi00138a003 , Abstract

Related entities 1. micrococcal nuclease, : 1 : 129 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr2784_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr2784_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	99		7.1 (chain: 1, length: 156)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 156, Sequence coverage: 7.1%
- Total number of assignments
  - ¹³C chemical shifts: 22
  - ¹H chemical shifts: 77
- Completeness of assignments
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts

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Found 1 Document (1.339 seconds)

BMRB: 2784

Sample

Related entities 1. micrococcal nuclease, : 1 : 129 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail