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Dynamics of Methyl Groups in Proteins As Studied by Proton-Detected 13C NMR Spectroscopy. Application to the Leucine Residues of Staphylococcal Nuclease
Authors
Nicholson, L.K., Kay, L.E., Baldisseri, D.M., Arango, J., Young, P.E., Bax, A., Torchia, D.A.
Assembly
micrococcal nuclease
Entity
1. micrococcal nuclease (polymer), 156 monomers, 17603.95 Da Detail

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ


Formula weight
17603.95 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 10.3 %, Completeness: 4.7 %, Completeness (bb): 2.1 % Detail

Polymer type: polypeptide(L)

Total1H13C
All 4.7 % (80 of 1698) 4.4 % (43 of 980) 5.2 % (37 of 718)
Backbone 2.1 % (16 of 763) 1.3 % (4 of 305) 2.6 % (12 of 458)
Sidechain 6.3 % (68 of 1081) 5.8 % (39 of 675) 7.1 % (29 of 406)
Aromatic 3.3 % (4 of 121) 0.0 % (0 of 61) 6.7 % (4 of 60)
Methyl27.9 % (43 of 154)28.6 % (22 of 77)27.3 % (21 of 77)

1. micrococcal nuclease

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Sample

Temperature 310 K, pH 7



Release date
1995-07-30
Citation
Dynamics of methyl groups in proteins as studied by proton-detected 13C NMR spectroscopy. Application to the leucine residues of staphylococcal nuclease
Nicholson, L.K., Kay, L.E., Baldisseri, D.M., Arango, J., Young, P.E., Bax, A., Torchia, D.A.
Biochemistry (1992), 31, 5253-5263, PubMed 1606149 , DOI 10.1021/bi00138a003 ,
Related entities 1. micrococcal nuclease, : 1 : 116 entities Detail