BMRBj - BMREntryMaster

Found 1 Document (0.627 seconds)

BMRB: 29

1POH

Two-Dimensional 1H NMR studies of Histidine-Containing Protein from Escherichia coli. 1. Sequential Resonance Assignments

Authors

Klevit, R.E., Drobny, G.P., Waygood, E.BRUCE.

Assembly

phosphocarrier protein HPr

Entity

1. phosphocarrier protein HPr (polymer), 85 monomers, 9119.249 Da Detail

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Formula weight

9119.249 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 63.6 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	63.6 % (314 of 494)	63.6 % (314 of 494)
Backbone	98.3 % (171 of 174)	98.3 % (171 of 174)
Sidechain	44.7 % (143 of 320)	44.7 % (143 of 320)
Aromatic	0.0 % (0 of 24)	0.0 % (0 of 24)
Methyl	20.0 % (11 of 55)	20.0 % (11 of 55)

1. phosphocarrier protein HPr

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Show sample condition

Sample

Temperature 303 K, pH 6.5

Release date

1995-07-30

Citation

Two-dimensional 1H NMR studies of histidine-containing protein from Escherichia coli. 1. Sequential resonance assignments
Klevit, R.E., Drobny, G.P., Waygood, E.BRUCE.
Biochemistry (1986), 25, 7760-7769, PubMed 3542034 , DOI 10.1021/bi00371a071 , Abstract

Related entities 1. phosphocarrier protein HPr, : 1 : 17 : 1 : 4 : 93 entities Detail

Interaction partners 1. phosphocarrier protein HPr, : 10 interactors Detail

More details for 10 interactors identified by 5 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr29_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr29_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	318		100.0 (chain: 1, length: 85)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 85, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 318
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.627 seconds)

BMRB: 29

Sample

Related entities 1. phosphocarrier protein HPr, : 1 : 17 : 1 : 4 : 93 entities Detail

Interaction partners 1. phosphocarrier protein HPr, : 10 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail