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Two-Dimensional 1H NMR studies of Histidine-Containing Protein from Escherichia coli. 1. Sequential Resonance Assignments
Authors
Klevit, R.E., Drobny, G.P., Waygood, E.BRUCE.
Assembly
phosphocarrier protein HPr
Entity
1. phosphocarrier protein HPr (polymer), 85 monomers, 9119.249 Da Detail

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE


Formula weight
9119.249 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 63.6 %, Completeness (bb): 99.4 % Detail

Polymer type: polypeptide(L)

Total1H
All63.6 % (314 of 494)63.6 % (314 of 494)
Backbone99.4 % (167 of 168)99.4 % (167 of 168)
Sidechain45.1 % (147 of 326)45.1 % (147 of 326)
Aromatic 0.0 % (0 of 24) 0.0 % (0 of 24)
Methyl27.3 % (15 of 55)27.3 % (15 of 55)

1. phosphocarrier protein HPr

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Sample

Temperature 303 K, pH 6.5



Release date
1995-07-30
Citation
Two-dimensional 1H NMR studies of histidine-containing protein from Escherichia coli. 1. Sequential resonance assignments
Klevit, R.E., Drobny, G.P., Waygood, E.BRUCE.
Biochemistry (1986), 25, 7760-7769, PubMed 3542034 , DOI 10.1021/bi00371a071 ,
Related entities 1. phosphocarrier protein HPr, : 1 : 15 : 1 : 4 : 89 entities Detail
Interaction partners 1. phosphocarrier protein HPr, : 10 interactors Detail
Experiments performed 1 experiments Detail