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Importance of Protein and DNA Phosphate Ester Backbone Flexibility in Protein-DNA Recognition
Authors
Wisniowski, P., Karslake, C., Piotto, M.E., Spangler, B.D., Moulin, A., Nikonowicz, E.P., Kaluarachchi, K., Gorenstein, D.G.
Assembly
lac repressor headpiece
Entity
1. lac repressor headpiece (polymer), 56 monomers, 6175.995 Da Detail

MKPVTLIDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQL


Formula weight
6175.995 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.9 %, Completeness: 77.7 %, Completeness (bb): 87.3 % Detail

Polymer type: polypeptide(L)

Total1H
All77.7 % (255 of 328)77.7 % (255 of 328)
Backbone87.3 % (96 of 110)87.3 % (96 of 110)
Sidechain69.3 % (151 of 218)69.3 % (151 of 218)
Aromatic71.4 % (10 of 14)71.4 % (10 of 14)
Methyl84.6 % (33 of 39)84.6 % (33 of 39)

1. lac repressor headpiece

MKPVTLIDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQL

Sample

Temperature 295 K, pH 6



Release date
1995-07-30
Citation
Importance of Protein and DNA Phosphate Ester Backbone Flexibility in Protein-DNA Recognition
Wisniowski, P., Karslake, C., Piotto, M.E., Spangler, B.D., Moulin, A., Nikonowicz, E.P., Kaluarachchi, K., Gorenstein, D.G.
Structure and Function (1992), 2, 17-54
Related entities 1. lac repressor headpiece, : 1 : 19 : 145 entities Detail
Interaction partners 1. lac repressor headpiece, : 6 interactors Detail
Experiments performed 1 experiments Detail