Solution NMR structure of the myristoylated feline immunodeficiency virus matrix protein
XGNGQGRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.9 % (1033 of 1417) | 65.6 % (481 of 733) | 81.0 % (439 of 542) | 79.6 % (113 of 142) |
Backbone | 84.7 % (625 of 738) | 79.8 % (206 of 258) | 88.3 % (317 of 359) | 84.3 % (102 of 121) |
Sidechain | 64.1 % (506 of 790) | 57.9 % (275 of 475) | 74.8 % (220 of 294) | 52.4 % (11 of 21) |
Aromatic | 45.8 % (44 of 96) | 47.9 % (23 of 48) | 41.3 % (19 of 46) | 100.0 % (2 of 2) |
Methyl | 79.4 % (100 of 126) | 77.8 % (49 of 63) | 81.0 % (51 of 63) |
1. entity 1
XGNGQGRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPH HHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details 50 mM sodium phosphate, 10 mM DTT, 5 mM sodium chloride, 4 g/L glucose, 1 g/L [U-99% 15N] ammonium chloride, 300 uM [U-15N] Feline Immunodeficiency Virus Myristoylated Matrix Protein, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 50 mM | |
2 | DTT | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 5 mM | |
4 | glucose | natural abundance | 4 g/L | |
5 | ammonium chloride | [U-99% 15N] | 1 g/L | |
6 | Feline Immunodeficiency Virus Myristoylated Matrix Protein | [U-15N] | 300 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details 50 mM sodium phosphate, 10 mM [U-98% 2H] DTT, 5 mM sodium chloride, 4 g/L [U-99% 13C] glucose, 1 g/L ammonium chloride, 300 uM [U-13C] Feline Immunodeficiency Virus Myristoylated Matrix Protein, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sodium phosphate | natural abundance | 50 mM | |
8 | DTT | [U-98% 2H] | 10 mM | |
9 | sodium chloride | natural abundance | 5 mM | |
10 | glucose | [U-99% 13C] | 4 g/L | |
11 | ammonium chloride | natural abundance | 1 g/L | |
12 | Feline Immunodeficiency Virus Myristoylated Matrix Protein | [U-13C] | 300 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details 50 mM sodium phosphate, 10 mM [U-98% 2H] DTT, 5 mM sodium chloride, 4 g/L [U-99% 13C] glucose, 1 g/L ammonium chloride, 50 mg/L Myristoyl coenzyme A, 300 uM [U-13C] Feline Immunodeficiency Virus Myristoylated Matrix Protein, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | sodium phosphate | natural abundance | 50 mM | |
14 | DTT | [U-98% 2H] | 10 mM | |
15 | sodium chloride | natural abundance | 5 mM | |
16 | glucose | [U-99% 13C] | 4 g/L | |
17 | ammonium chloride | natural abundance | 1 g/L | |
18 | Feline Immunodeficiency Virus Myristoylated Matrix Protein | [U-13C] | 300 uM | |
19 | Myristoyl coenzyme A | natural abundance | 50 mg/L |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details 50 mM sodium phosphate, 10 mM DTT, 5 mM sodium chloride, 4 g/L [U-99% 13C] glucose, 1 g/L [U-99% 15N] ammonium chloride, 300 uM [U-13C; U-15N] Feline Immunodeficiency Virus Myristoylated Matrix Protein, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | sodium phosphate | natural abundance | 50 mM | |
21 | DTT | natural abundance | 10 mM | |
22 | sodium chloride | natural abundance | 5 mM | |
23 | glucose | [U-99% 13C] | 4 g/L | |
24 | ammonium chloride | [U-99% 15N] | 1 g/L | |
25 | Feline Immunodeficiency Virus Myristoylated Matrix Protein | [U-13C; U-15N] | 300 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details 50 mM sodium phosphate, 10 mM [U-98% 2H] DTT, 5 mM sodium chloride, 4 g/L glucose, 1 g/L ammonium chloride, 300 uM Feline Immunodeficiency Virus Myristoylated Matrix Protein, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | sodium phosphate | natural abundance | 50 mM | |
27 | DTT | [U-98% 2H] | 10 mM | |
28 | sodium chloride | natural abundance | 5 mM | |
29 | glucose | natural abundance | 4 g/L | |
30 | ammonium chloride | natural abundance | 1 g/L | |
31 | Feline Immunodeficiency Virus Myristoylated Matrix Protein | natural abundance | 300 uM |