BMRB: 34110

Structure of the N-terminal domain of the Escherichia Coli ProQ RNA binding protein

Authors

Gonzales, G., Hardwick, S., Maslen, S., Skehel, M., Holmqvist, E., Vogel, J., Bateman, A., Luisi, B., Broadhurst, R.

Assembly

RNA chaperone ProQ

Entity

1. RNA chaperone ProQ (polymer), 133 monomers, 15081.69 Da Detail

MGSSHHHHHH SQDPMENQPK LNSSKEVIAF LAERFPHCFS AEGEARPLKI GIFQDLVDRV AGEMNLSKTQ LRSALRLYTS SWRYLYGVKP GATRVDLDGN PCGELDEQHV EHARKQLEEA KARVQAQRAE QQA

Formula weight

15081.69 Da

Source organism

Escherichia coli S88

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 80.9 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.9 % (1246 of 1541)	76.4 % (619 of 810)	85.1 % (501 of 589)	88.7 % (126 of 142)
Backbone	91.5 % (719 of 786)	91.0 % (244 of 268)	91.3 % (357 of 391)	92.9 % (118 of 127)
Sidechain	73.1 % (643 of 880)	69.2 % (375 of 542)	80.5 % (260 of 323)	53.3 % (8 of 15)
Aromatic	68.8 % (77 of 112)	71.4 % (40 of 56)	65.5 % (36 of 55)	100.0 % (1 of 1)
Methyl	94.2 % (113 of 120)	95.0 % (57 of 60)	93.3 % (56 of 60)

1. entity 1

MGSSHHHHHH SQDPMENQPK LNSSKEVIAF LAERFPHCFS AEGEARPLKI GIFQDLVDRV AGEMNLSKTQ LRSALRLYTS SWRYLYGVKP GATRVDLDGN PCGELDEQHV EHARKQLEEA KARVQAQRAE QQA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance		20 (±1.0) mM
3	sodium chloride	natural abundance		100 (±5.0) mM
4	TCEP	natural abundance		1 (±0.1) mM
5	3,3,3-trimethylsilylpropionate	natural abundance		0.0025 (±5.0E-4) %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Type	Concentration
6	entity_1	natural abundance		0.0 ~ 0.0 mM
7	sodium phosphate	natural abundance		20 (±1.0) mM
8	sodium chloride	natural abundance		100 (±5.0) mM
9	TCEP	natural abundance		1 (±0.1) mM
10	3,3,3-trimethylsilylpropionate	natural abundance		0.0025 (±5.0E-4) %

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LACS Plot; CA

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	internal	direct	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	internal	direct	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	internal	direct	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	internal	direct	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 17 models in PDB: 5NB9, Strand ID: A Detail

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Release date

2017-05-01

Citation

Structure of the Escherichia coli ProQ RNA-binding protein
Gonzalez, G.M., Hardwick, S.W., Maslen, S.L., Skehel, J.M., Holmqvist, E., Vogel, J., Bateman, A., Luisi, B.F., Broadhurst, R.W.
RNA (2017), 23, 696-711, PubMed 28193673 , DOI 10.1261/rna.060343.116 , Abstract

Related entities 1. RNA chaperone ProQ, : 9 : 41 entities Detail

Interaction partners 1. RNA chaperone ProQ, : 43 interactors Detail

Experiments performed 11 experiments Detail