BMRBj - BMREntryMaster

Found 1 Document (0.937 seconds)

BMRB: 34126

5NR5

NMR structure and 1H, 13C and 15N signal assignments for Dictyostelium discoideum MATA protein

Authors

Neuhaus, D., Hedgethorne, K., Yang, J.

Assembly

MatA protein

Entity

1. MatA protein (polymer, Thiol state: not present), 113 monomers, 12494.31 Da Detail

GSHMASMDPL DKIINDIKKE ANDSGVTLAP LSVPKPKLEE LSEQQKIILA EYIAEVGLQN ITAITLSKKL NITVEKAKNY IKNSNRLGRT NNLKTIGILQ EEVSSMEAKS MTW

Formula weight

12494.31 Da

Source organism

Dictyostelium discoideum

Exptl. method

solution NMR

Refine. method

simulated annealingsimulated annealingmolecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.9 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.9 % (1198 of 1332)	97.3 % (679 of 698)	78.1 % (399 of 511)	97.6 % (120 of 123)
Backbone	82.7 % (554 of 670)	96.9 % (220 of 227)	68.3 % (228 of 334)	97.2 % (106 of 109)
Sidechain	97.5 % (751 of 770)	97.5 % (459 of 471)	97.5 % (278 of 285)	100.0 % (14 of 14)
Aromatic	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (15 of 15)	100.0 % (1 of 1)
Methyl	97.3 % (142 of 146)	95.9 % (70 of 73)	98.6 % (72 of 73)

1. entity 1

GSHMASMDPL DKIINDIKKE ANDSGVTLAP LSVPKPKLEE LSEQQKIILA EYIAEVGLQN ITAITLSKKL NITVEKAKNY IKNSNRLGRT NNLKTIGILQ EEVSSMEAKS MTW

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 7.0, Details 600 uM [U-15N] MATA, 50 mM [U-2H] TRIS, 100 mM sodium chloride, 1 mM [U-2H] DTT, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	DTT	[U-2H]	1 mM
2	EDTA	natural abundance	50 uM
3	MATA	[U-15N]	600 (±100.0) uM
4	TRIS	[U-2H]	50 mM
5	sodium chloride	natural abundance	100 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 7.0, Details 600 uM [U-13C; U-15N] MATA, 50 mM [U-2H] TRIS, 100 mM sodium chloride, 1 mM [U-2H] DTT, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 5NR5, Strand ID: A Detail

Release date

2017-04-23

Citation

Homeodomain-like DNA binding proteins control the haploid-to-diploid transition in Dictyostelium
Hedgethorne, K., Eustermann, S., Yang, J.C., Ogden, T., Neuhaus, D., Bloomfield, G.
Sci. Adv. (2017), 3, e1602937-e1602937, PubMed 28879231 , DOI 10.1126/sciadv.1602937 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34127 released on 2017-05-07
Title NMR structure and 1H, 13C and 15N signal assignments for Dictyostelium discoidans MATB protein S71A mutant

Related entities 1. MatA protein, : 1 : 1 : 4 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 7.0, Details 600 uM [U-15N] MATA, 50 mM [U-2H] TRIS, 100 mM sodium chloride, 1 mM [U-2H] DTT, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	DTT	[U-2H]	1 mM
2	EDTA	natural abundance	50 uM
3	MATA	[U-15N]	600 (±100.0) uM
4	TRIS	[U-2H]	50 mM
5	sodium chloride	natural abundance	100 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 7.0, Details 600 uM [U-13C; U-15N] MATA, 50 mM [U-2H] TRIS, 100 mM sodium chloride, 1 mM [U-2H] DTT, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

4 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

5 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

6 3D HNHAHB

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

7 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

8 3D [1H-13C-1H] HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

9 3D [13C-13C-1H] HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

10 3D [1H-13C-1H] HCCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

11 3D 1H-15N NOESY (mix. time 150ms)

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

12 3D 1H-13C NOESY aliphatic (mix. time 150ms)

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

13 3D 1H-13C NOESY aromatic (mix. time 150ms)

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	[U-2H]	1 mM
7	EDTA	natural abundance	50 uM
8	MATA	[U-13C; U-15N]	600 (±100.0) uM
9	TRIS	[U-2H]	50 mM
10	sodium chloride	natural abundance	100 mM

nullKeywords DNA binding protein, Homeobox, Mating type determination factor

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Found 1 Document (0.937 seconds)

BMRB: 34126

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. MatA protein, : 1 : 1 : 4 entities Detail

Experiments performed 13 experiments Detail

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