BMRB: 3524

13C NMR Determination of the Tautomeric and Ionization States of Folate in Its Complexes with Lactobacillus casei Dihydrofolate Reductase

Authors

Cheung, H.T.A., Birdsall, B., Frenkiel, T.A., Chau, D.D., Feeney, J.

Assembly

dihydrofolate reductase

Entity

1. dihydrofolate reductase (polymer), 162 monomers, 18307.38 Da Detail

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

Formula weight

18307.38 Da

Source organism

Lacticaseibacillus casei

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 6.2 %, Completeness: 2.4 %, Completeness (bb): 1.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	2.4 % (23 of 971)	2.4 % (23 of 971)
Backbone	1.8 % (6 of 326)	1.8 % (6 of 326)
Sidechain	2.6 % (17 of 645)	2.6 % (17 of 645)
Aromatic	9.2 % (9 of 98)	9.2 % (9 of 98)
Methyl	5.2 % (5 of 97)	5.2 % (5 of 97)

1. dihydrofolate reductase

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

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Sample

Temperature 308 K, pH 5.3

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Release date

1995-07-30

Citation

13C NMR determination of the tautomeric and ionization states of folate in its complexes with Lactobacillus casei dihydrofolate reductase
Cheung, H.T.A., Birdsall, B., Frenkiel, T.A., Chau, D.D., Feeney, J.
Biochemistry (1993), 32, 6846-6854, PubMed 8334117 , DOI: , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. dihydrofolate reductase, : 1 : 9 : 137 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail