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Resonance Assignments for the OB-fold Sub-Domain of Staphylococcal Nuclease (SNOB) -> Nuclease 1-103 (V66L, G88V)
Authors
Alexandrescu, A., Gittis, A., Abeygunawardana, C., Shortle, D.
Assembly
OB-fold sub-domain of staphylococcal nuclease
Entity
1. OB-fold sub-domain of staphylococcal nuclease (polymer), 103 monomers, 11604.40 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMLENAK KIEVEFDKGQ RTDKYGRVLA YIYADGKMVN EAL


Formula weight
11604.4 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 99.0 %, Completeness: 88.1 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All88.1 % (667 of 757)86.3 % (563 of 652)99.0 % (104 of 105)
Backbone98.7 % (295 of 299)98.5 % (198 of 201)99.0 % (97 of 98)
Sidechain81.2 % (372 of 458)80.9 % (365 of 451)100.0 % (7 of 7)
Aromatic87.2 % (34 of 39)87.2 % (34 of 39)
Methyl94.6 % (53 of 56)94.6 % (53 of 56)

1. SNOB

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMLENAK KIEVEFDKGQ RTDKYGRVLA YIYADGKMVN EAL

Sample

Temperature 305 K, pH 4.7



Chem. Shift Complete2
Sequence coverage: 24.3 %, Completeness: 51.1 %, Completeness (bb): 59.2 % Detail

Polymer type: polypeptide(L)

Total1H15N
All51.1 % (773 of 1514)49.5 % (646 of 1304)60.5 % (127 of 210)
Backbone59.2 % (354 of 598)58.0 % (233 of 402)61.7 % (121 of 196)
Sidechain45.9 % (420 of 916)45.8 % (413 of 902)50.0 % (7 of 14)
Aromatic51.3 % (40 of 78)51.3 % (40 of 78)
Methyl53.6 % (60 of 112)53.6 % (60 of 112)

1. SNOB

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMLENAK KIEVEFDKGQ RTDKYGRVLA YIYADGKMVN EAL

Sample

Temperature 305 K, pH 4.7



Chem. Shift Complete3
Sequence coverage: 14.6 %, Completeness: 36.9 %, Completeness (bb): 43.5 % Detail

Polymer type: polypeptide(L)

Total1H15N
All36.9 % (837 of 2271)35.5 % (695 of 1956)45.1 % (142 of 315)
Backbone43.5 % (390 of 897)42.3 % (255 of 603)45.9 % (135 of 294)
Sidechain32.7 % (449 of 1374)32.6 % (441 of 1353)38.1 % (8 of 21)
Aromatic34.2 % (40 of 117)34.2 % (40 of 117)
Methyl38.7 % (65 of 168)38.7 % (65 of 168)

1. SNOB

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMLENAK KIEVEFDKGQ RTDKYGRVLA YIYADGKMVN EAL

Sample

Temperature 305 K, pH 4.7



Protein Blocks Logo
Calculated from 10 models in PDB: 2SOB, Strand ID: A Detail


Release date
1998-07-04
Citation
NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease
Alexandrescu, A., Gittis, A., Abeygunawardana, C., Shortle, D.
J. Mol. Biol. (1995), 250, 134-143, PubMed 7608966 , DOI 10.1006/jmbi.1995.0365 ,
Related entities 1. OB-fold sub-domain of staphylococcal nuclease, : 1 : 11 : 102 entities Detail
Keywords evolution, NMR, OB-fold, protein folding, protein structure, SNOB, OB-fold sub-domain of staphylococcal nuclease