BMRBj - BMREntryMaster

Found 1 Document (1.383 seconds)

BMRB: 4012

1F21

Structure of the Acid State of E. coli Ribonuclease HI

Authors

Dabora, J.M., Pelton, J.G., Marqusee, S.

Assembly

Ribonuclease H1

Entity

1. Ribonuclease H1 (polymer, Thiol state: not present), 155 monomers, 17500.58 Da Detail

MLKQVEIFTD GSALGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHAEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERADELARAA AMNPTLEDTG YQVEV

Formula weight

17500.58 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.7 %, Completeness: 33.2 %, Completeness (bb): 62.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.2 % (602 of 1812)	32.6 % (308 of 944)	22.1 % (154 of 697)	81.9 % (140 of 171)
Backbone	62.1 % (571 of 920)	87.5 % (279 of 319)	33.9 % (153 of 451)	92.7 % (139 of 150)
Sidechain	3.8 % (39 of 1033)	4.6 % (29 of 625)	2.3 % (9 of 387)	4.8 % (1 of 21)
Aromatic	0.0 % (0 of 152)	0.0 % (0 of 76)	0.0 % (0 of 70)	0.0 % (0 of 6)
Methyl	7.2 % (12 of 166)	9.6 % (8 of 83)	4.8 % (4 of 83)

1. Ribonuclease H1

MLKQVEIFTD GSALGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHAEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERADELARAA AMNPTLEDTG YQVEV

Show sample condition

Sample

Temperature 298 (±1.0) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribonuclease H1	[U-99% 13C; U-99% 15N]		1.0 ~ 3.0 mM

Release date

2001-02-15

Citation

Structure of the acid state of Escherichia coli ribonuclease HI
Dabora, J.M., Pelton, J.G., Marqusee, S.
Biochemistry (1996), 35, 11951-11958, PubMed 8810899 , DOI 10.1021/bi9611671 , Abstract

Related entities 1. Ribonuclease H1, : 1 : 32 : 182 entities Detail

Interaction partners 1. Ribonuclease H1, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4012_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4012_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	Warning	552		89.0 (chain: 1, length: 155)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 155, Sequence coverage: 89.0%
- Total number of assignments
  - ¹H chemical shifts: 276
  - ¹⁵N chemical shifts: 138
  - ¹³C chemical shifts: 138
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	944	276	29.2
¹⁵N chemical shifts	181	138	76.2
¹³C chemical shifts	697	138	19.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	319	276	86.5
¹⁵N chemical shifts	150	138	92.0
¹³C chemical shifts	310	138	44.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	625	0	0.0
¹⁵N chemical shifts	31	0	0.0
¹³C chemical shifts	387	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	0	0.0
¹³C chemical shifts	87	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	76	0	0.0
¹⁵N chemical shifts	6	0	0.0
¹³C chemical shifts	70	0	0.0

Keywords protein folding

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Found 1 Document (1.383 seconds)

BMRB: 4012

Sample

Related entities 1. Ribonuclease H1, : 1 : 32 : 182 entities Detail

Interaction partners 1. Ribonuclease H1, : 7 interactors Detail

Chemical shift validation 3 contents Detail