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Structure of the Acid State of E. coli Ribonuclease HI
Authors
Dabora, J.M., Pelton, J.G., Marqusee, S.
Assembly
Ribonuclease H1
Entity
1. Ribonuclease H1 (polymer, Thiol state: not present), 155 monomers, 17500.58 Da Detail

MLKQVEIFTD GSALGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHAEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERADELARAA AMNPTLEDTG YQVEV


Formula weight
17500.58 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 91.0 %, Completeness: 35.1 %, Completeness (bb): 63.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All35.1 % (640 of 1822)34.0 % (321 of 944)25.5 % (178 of 697)77.9 % (141 of 181)
Backbone63.2 % (573 of 906)88.2 % (269 of 305)36.1 % (163 of 451)94.0 % (141 of 150)
Sidechain 7.5 % (79 of 1057) 8.1 % (52 of 639) 7.0 % (27 of 387) 0.0 % (0 of 31)
Aromatic 0.0 % (0 of 152) 0.0 % (0 of 76) 0.0 % (0 of 70) 0.0 % (0 of 6)
Methyl 9.0 % (15 of 166) 9.6 % (8 of 83) 8.4 % (7 of 83)

1. Ribonuclease H1

MLKQVEIFTD GSALGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHAEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERADELARAA AMNPTLEDTG YQVEV

Sample

Temperature 298 (±1.0) K, pH 5.5 (±0.1)


#NameIsotope labelingTypeConcentration
1Ribonuclease H1[U-99% 13C; U-99% 15N]1.0 ~ 3.0 mM

Release date
2001-02-15
Citation
Structure of the acid state of Escherichia coli ribonuclease HI
Dabora, J.M., Pelton, J.G., Marqusee, S.
Biochemistry (1996), 35, 11951-11958, PubMed 8810899 , DOI 10.1021/bi9611671 ,
Related entities 1. Ribonuclease H1, : 1 : 26 : 172 entities Detail
Interaction partners 1. Ribonuclease H1, : 5 interactors Detail
Keywords protein folding