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BMRB: 4024

1H Resonance Assignments for the First Zinc Finger Domain of SWI5

Authors

Dutnall, R.N., Neuhaus, D., Rhodes, D.

Assembly

The first zinc finger domain of SWI5

Entity

1. The first zinc finger domain of SWI5 (polymer), 59 monomers, 6984.994 Da Detail

TNSPSKITRK LTTLPRGSID KYVKEMPDKT FECLFPGCTK TFKRRYNIRS HIQTHLEDR

2. zinc (non-polymer), 1 monomers, 65.409 Da

Total weight

7050.4033 Da

Max. entity weight

6984.994 Da

Entity Connection

metal coordination 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS33:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS38:SG	2:ZN1:ZN
3	metal coordination	sing	1:HIS51:NE2	2:ZN1:ZN
4	metal coordination	sing	1:HIS55:NE2	2:ZN1:ZN

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.6 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	95.6 % (370 of 387)	95.6 % (370 of 387)
Backbone	96.6 % (112 of 116)	96.6 % (112 of 116)
Sidechain	95.2 % (258 of 271)	95.2 % (258 of 271)
Aromatic	92.6 % (25 of 27)	92.6 % (25 of 27)
Methyl	100.0 % (26 of 26)	100.0 % (26 of 26)

1. The first zinc finger domain of SWI5

TNSPSKITRK LTTLPRGSID KYVKEMPDKT FECLFPGCTK TFKRRYNIRS HIQTHLEDR

Show sample condition

Sample

Temperature 283 K, pH 6.5

#	Name	Concentration
1	The first zinc finger domain of SWI5	4 mM
2	sodium pyrophosphate buffer	40 mM
3	zinc sulphate	0.5 mM
4	TSP	0.1 mM
5	D2O	15 %
6	H2O	85 %

Protein Blocks Logo

Calculated from 46 models in PDB: 1NCS, Strand ID: A Detail

Release date

1999-10-11

Citation

The solution structure of the first zinc finger domain of SWI5: a novel structural extension to a common fold
Dutnall, R.N., Neuhaus, D., Rhodes, D.
Structure (1996), 4, 599-611, PubMed 8736557 , DOI: , Abstract

Related entities 1. The first zinc finger domain of SWI5, : 2 : 34 entities Detail

Interaction partners 1. The first zinc finger domain of SWI5, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4024_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:33:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:38:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:51:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a
1:55:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

None

Content subtype: bmr4024_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	Warning	430		100.0 (chain: 1, length: 59)
1	Chemical shift references	chemical_shift_reference_one	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 59, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 430
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
51	HIS	HE2	7.84
55	HIS	HD1	12.2
55	HIS	HE2	7.99

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	387	373	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	116	113	97.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	271	260	95.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	27	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	25	92.6

Keywords NMR structure, protein-DNA recognition, zinc finger

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