BMRBj - BMREntryMaster

Found 1 Document (1.146 seconds)

BMRB: 4035

1DHM

Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions

Authors

Liang, H., Petros, A.M., Meadows, R.P., Yoon, H.S., Egan, D.A., Walter, K., Holzman, T.F., Robins, T., Fesik, S.W.

Assembly

Regulatory protein E2

Entity

1. Regulatory protein E2 (polymer), 83 monomers, 9510.860 Da Detail

MATTPIIHLK GDANILKCLR YRLSKYKQLY EQVSSTWHWT CTDGKHKNAI VTLTYISTSQ RDDFLNTVKI PNTVSVSTGY MTI

Formula weight

9510.86 Da

Source organism

Human papillomavirus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.9 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.9 % (868 of 987)	94.5 % (479 of 507)	77.9 % (304 of 390)	94.4 % (85 of 90)
Backbone	81.8 % (404 of 494)	94.6 % (158 of 167)	68.7 % (169 of 246)	95.1 % (77 of 81)
Sidechain	94.6 % (542 of 573)	94.4 % (321 of 340)	95.1 % (213 of 224)	88.9 % (8 of 9)
Aromatic	95.3 % (82 of 86)	95.3 % (41 of 43)	95.1 % (39 of 41)	100.0 % (2 of 2)
Methyl	99.1 % (105 of 106)	98.1 % (52 of 53)	100.0 % (53 of 53)

1. Regulatory protein E2

MATTPIIHLK GDANILKCLR YRLSKYKQLY EQVSSTWHWT CTDGKHKNAI VTLTYISTSQ RDDFLNTVKI PNTVSVSTGY MTI

Show sample condition

Sample #1

Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Regulatory protein E2	[U-15N;U-13C]	1.7 mM
2	D2O		10 %
3	H2O		90 %
4	sodium_phosphate		20 mM
5	dithiothreitol		5 mM

Sample #2

Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	Regulatory protein E2	[U-15N;U-13C]	1.7 mM
7	D2O		100 %
8	sodium_phosphate		20 mM
9	dithiothreitol		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 1.71 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 1.71 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1DHM, Strand ID: A, B Detail

Release date

1997-03-01

Citation

Solution structure of the DNA-binding domain of a human papillomavirus E2 protein: evidence for flexible DNA-binding regions
Liang, H., Petros, A.M., Meadows, R.P., Yoon, H.S., Egan, D.A., Walter, K., Holzman, T.F., Robins, T., Fesik, S.W.
Biochemistry (1996), 35, 2095-2103, PubMed 8652551 , DOI 10.1021/bi951932w , Abstract

Related entities 1. Regulatory protein E2, : 1 : 2 : 91 entities Detail

Interaction partners 1. Regulatory protein E2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4035_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4035_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	Warning	979		100.0 (chain: 1, length: 83)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 83, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 599
  - ¹³C chemical shifts: 296
  - ¹⁵N chemical shifts: 84
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	507	491	96.8
¹³C chemical shifts	390	296	75.9
¹⁵N chemical shifts	93	84	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	167	161	96.4
¹³C chemical shifts	166	83	50.0
¹⁵N chemical shifts	81	76	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	340	330	97.1
¹³C chemical shifts	224	213	95.1
¹⁵N chemical shifts	12	8	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	54	98.2
¹³C chemical shifts	55	54	98.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	41	95.3
¹³C chemical shifts	41	39	95.1
¹⁵N chemical shifts	2	2	100.0

Keywords Human Papillomavirus (HPV), NMR, Nuclear Magnetic Resonance

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Found 1 Document (1.146 seconds)

BMRB: 4035

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Regulatory protein E2, : 1 : 2 : 91 entities Detail

Interaction partners 1. Regulatory protein E2, : 1 interactors Detail

Chemical shift validation 3 contents Detail