BMRBj - BMREntryMaster

Found 1 Document (1.231 seconds)

BMRB: 4044

4LYL

Secondary Structure of Uracil-DNA Glycosylase Inhibitor Protein

Authors

Balasubramanian, S., Beger, R.D., Bennett, S.E., Mosbaugh, D.W., Bolton, P.H.

Assembly

Uracil-DNA Glycosylase Inhibitor Protein

Entity

1. Uracil-DNA Glycosylase Inhibitor Protein (polymer), 84 monomers, 9475.613 Da Detail

MTNLSDIIEK ETGKQLVIQE SILMLPEEVE EVIGNKPESD ILVHTAYDES TDENVMLLTS DAPEYKPWAL VIQDSNGENK IKML

Formula weight

9475.613 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 85.8 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	85.8 % (513 of 598)	85.5 % (435 of 509)	87.6 % (78 of 89)
Backbone	96.4 % (238 of 247)	95.8 % (160 of 167)	97.5 % (78 of 80)
Sidechain	78.3 % (275 of 351)	80.4 % (275 of 342)	0.0 % (0 of 9)
Aromatic	94.1 % (16 of 17)	100.0 % (16 of 16)	0.0 % (0 of 1)
Methyl	77.8 % (42 of 54)	77.8 % (42 of 54)

1. Uracil-DNA Glycosylase Inhibitor Protein

MTNLSDIIEK ETGKQLVIQE SILMLPEEVE EVIGNKPESD ILVHTAYDES TDENVMLLTS DAPEYKPWAL VIQDSNGENK IKML

Show sample condition

Sample

Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Uracil-DNA_Glycosylase_Inhibitor_Protein	[U-15N]	3 mM
2	deuterated_Tris		25 mM
3	EDTA		0.2 mM
4	EGTA		0.2 mM
5	NaCl		100 mM

Release date

2001-02-15

Citation

Secondary structure of uracil-DNA glycosylase inhibitor protein
Balasubramanian, S., Beger, R.D., Bennett, S.E., Mosbaugh, D.W., Bolton, P.H.
J. Biol. Chem. (1995), 270, 296-303, PubMed 7814390 , DOI: , Abstract

Related entities 1. Uracil-DNA Glycosylase Inhibitor Protein, : 1 : 10 : 4 : 2 entities Detail

Interaction partners 1. Uracil-DNA Glycosylase Inhibitor Protein, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4044_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4044_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_one	Warning	586		94.0 (chain: 1, length: 84)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 84, Sequence coverage: 94.0%
- Total number of assignments
  - ¹H chemical shifts: 510
  - ¹⁵N chemical shifts: 76
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
44	HIS	HE2	10.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	509	429	84.3
¹⁵N chemical shifts	89	76	85.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	167	158	94.6
¹⁵N chemical shifts	80	76	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	342	271	79.2
¹⁵N chemical shifts	9	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	40	69.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	16	100.0
¹⁵N chemical shifts	1	0	0.0

Keywords NMR, Nuclear Magnetic Resonance, B. subtilis, Bacteriophage, PBS2, Uracil-DNA Glycosylase Inhibitor Protein (Ugi)

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Found 1 Document (1.231 seconds)

BMRB: 4044

Sample

Related entities 1. Uracil-DNA Glycosylase Inhibitor Protein, : 1 : 10 : 4 : 2 entities Detail

Interaction partners 1. Uracil-DNA Glycosylase Inhibitor Protein, : 3 interactors Detail

Chemical shift validation 3 contents Detail