BMRB: 4046

NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors

Authors

Vuister, G.W., Kim, S., Wu, C., Bax, A.

Assembly

Drosophila Heat Shock Factor

Entity

1. Drosophila Heat Shock Factor (polymer), 136 monomers, 15400.23 Da Detail

AAEHSYGDAA AIGSGVPAFL AKLWRIVDDA DTNRLICWTK DGQSFVIQNQ AQFAKELLPL NYKHNNMASF IRQLNMYGFH KITSIDNGGL RFDRDEIEFS HPFFKRNSPF LLDQIKRKIS NNKNGDDKGV LKPAAE

Formula weight

15400.23 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 59.3 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	59.3 % (963 of 1624)	53.4 % (453 of 849)	61.9 % (387 of 625)	82.0 % (123 of 150)
Backbone	93.9 % (757 of 806)	94.6 % (261 of 276)	93.5 % (373 of 399)	93.9 % (123 of 131)
Sidechain	34.5 % (326 of 945)	33.5 % (192 of 573)	38.0 % (134 of 353)	0.0 % (0 of 19)
Aromatic	0.0 % (0 of 164)	0.0 % (0 of 82)	0.0 % (0 of 80)	0.0 % (0 of 2)
Methyl	29.5 % (39 of 132)	28.8 % (19 of 66)	30.3 % (20 of 66)

1. Drosophila Heat Shock Factor

AAEHSYGDAA AIGSGVPAFL AKLWRIVDDA DTNRLICWTK DGQSFVIQNQ AQFAKELLPL NYKHNNMASF IRQLNMYGFH KITSIDNGGL RFDRDEIEFS HPFFKRNSPF LLDQIKRKIS NNKNGDDKGV LKPAAE

Show sample condition

Sample #1

Temperature 300 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	Drosophila Heat Shock Factor	[U-15N]		0.7 ~ 1.1 mM
2	D2O			5 %
3	potassium phosphate			10 mM
4	KCl			50 mM

Sample #2

Temperature 300 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
5	Drosophila Heat Shock Factor	[U-13C; U-15N]		0.7 ~ 1.1 mM
6	D2O			5 %
7	potassium phosphate			10 mM
8	KCl			50 mM

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	TSP	methyl protons	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	TSP	methyl protons	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	TSP	methyl protons	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	TSP	methyl protons	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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Release date

1999-08-23

Citation

NMR evidence for similarities between the DNA-binding regions of Drosophila melanogaster heat shock factor and the helix-turn-helix and HNF-3/forkhead families of transcription factors
Vuister, G.W., Kim, S., Wu, C., Bax, A.
Biochemistry (1994), 33, 10-16, PubMed 8286326 , DOI: , Abstract

Related entities 1. Drosophila Heat Shock Factor, : 83 entities Detail

Interaction partners 1. Drosophila Heat Shock Factor, : 4 interactors Detail

Chemical shift validation 3 contents Detail