NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors
AAEHSYGDAA AIGSGVPAFL AKLWRIVDDA DTNRLICWTK DGQSFVIQNQ AQFAKELLPL NYKHNNMASF IRQLNMYGFH KITSIDNGGL RFDRDEIEFS HPFFKRNSPF LLDQIKRKIS NNKNGDDKGV LKPAAE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.3 % (963 of 1624) | 53.4 % (453 of 849) | 61.9 % (387 of 625) | 82.0 % (123 of 150) |
Backbone | 93.9 % (757 of 806) | 94.6 % (261 of 276) | 93.5 % (373 of 399) | 93.9 % (123 of 131) |
Sidechain | 34.5 % (326 of 945) | 33.5 % (192 of 573) | 38.0 % (134 of 353) | 0.0 % (0 of 19) |
Aromatic | 0.0 % (0 of 164) | 0.0 % (0 of 82) | 0.0 % (0 of 80) | 0.0 % (0 of 2) |
Methyl | 29.5 % (39 of 132) | 28.8 % (19 of 66) | 30.3 % (20 of 66) |
1. Drosophila Heat Shock Factor
AAEHSYGDAA AIGSGVPAFL AKLWRIVDDA DTNRLICWTK DGQSFVIQNQ AQFAKELLPL NYKHNNMASF IRQLNMYGFH KITSIDNGGL RFDRDEIEFS HPFFKRNSPF LLDQIKRKIS NNKNGDDKGV LKPAAETemperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Drosophila Heat Shock Factor | [U-15N] | 0.7 ~ 1.1 mM | |
2 | D2O | 5 % | ||
3 | potassium phosphate | 10 mM | ||
4 | KCl | 50 mM |
Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Drosophila Heat Shock Factor | [U-13C; U-15N] | 0.7 ~ 1.1 mM | |
6 | D2O | 5 % | ||
7 | potassium phosphate | 10 mM | ||
8 | KCl | 50 mM |