BMRB: 4049

Solution Structure of the Amino-Terminal Fragment of Urokinase-Type Plasminogen Activator

Authors

Hansen, A.P., Petros, A.M., Meadows, R.P., Nettesheim, D.G., Mazar, A.P., Olejniczak, E.T., Xu, R.X., Pederson, T.M., Henkin, J., Fesik, S.W.

Assembly

Urokinase-type plasminogen activator

Entity

1. u-PA amino-terminal fragment (polymer), 130 monomers, 14696.48 Da Detail

QVPSNCDCLN GGTCVSNKYF SNIHWCNCPK KFGGQHCEID KSKTCYEGNG HFYRGKASTD TMGRPCLPWN SATVLQQTYH AHRSDALQLG LGKHNYCRNP DNRRRPWCYV QVGLKPLVQE CMVHDCADGK

Formula weight

14696.48 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.6 %, Completeness (bb): 80.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.6 % (1275 of 1489)	93.2 % (728 of 781)	74.7 % (422 of 565)	87.4 % (125 of 143)
Backbone	80.5 % (617 of 766)	96.2 % (255 of 265)	64.6 % (244 of 378)	95.9 % (118 of 123)
Sidechain	90.8 % (764 of 841)	91.7 % (473 of 516)	93.1 % (284 of 305)	35.0 % (7 of 20)
Aromatic	93.0 % (132 of 142)	95.8 % (68 of 71)	91.2 % (62 of 68)	66.7 % (2 of 3)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. u-PA amino-terminal fragment

QVPSNCDCLN GGTCVSNKYF SNIHWCNCPK KFGGQHCEID KSKTCYEGNG HFYRGKASTD TMGRPCLPWN SATVLQQTYH AHRSDALQLG LGKHNYCRNP DNRRRPWCYV QVGLKPLVQE CMVHDCADGK

Show sample condition

Sample

Temperature 303 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
5	u-PA amino-terminal fragment	[U-13C; U-15N]		1.0 mM
6	D2O			10 %
7	H2O			90 %
8	sodium acetate-d3

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LACS Plot; CA

Referencing offset: 0.35 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	H2O	protons	4.74 ppm	null	null	0.0
15N	NH4NO3	na	119.4 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.35 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	H2O	protons	4.74 ppm	null	null	0.0
15N	NH4NO3	na	119.4 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 6 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl carbons	0.0 ppm	null	null	0.0
1H	H2O	protons	4.74 ppm	null	null	0.0
15N	NH4NO3	na	119.4 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 15 models in PDB: 1URK, Strand ID: A Detail

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Release date

1999-08-16

Citation

Solution structure of the amino-terminal fragment of urokinase-type plasminogen activator
Hansen, A.P., Petros, A.M., Meadows, R.P., Nettesheim, D.G., Mazar, A.P., Olejniczak, E.T., Xu, R.X., Pederson, T.M., Henkin, J., Fesik, S.W.
Biochemistry (1994), 33, 4847-4864, PubMed 8161544 , DOI: , Abstract

Related entities 1. u-PA amino-terminal fragment, : 1 : 4 : 6 : 43 entities Detail

Interaction partners 1. u-PA amino-terminal fragment, : 9 interactors Detail