Solution structures of Staphylococcal Nuclease from multidimensional, multinuclear NMR: nuclease-H124L and its ternary complex with Ca2+ and Thymidine-3'-5'-bisphosphate
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDNADSGQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.8 % (1548 of 1783) | 90.3 % (849 of 940) | 82.1 % (564 of 687) | 86.5 % (135 of 156) |
Backbone | 91.6 % (808 of 882) | 92.1 % (278 of 302) | 91.5 % (400 of 437) | 90.9 % (130 of 143) |
Sidechain | 83.1 % (864 of 1040) | 89.5 % (571 of 638) | 73.8 % (287 of 389) | 46.2 % (6 of 13) |
Aromatic | 94.5 % (104 of 110) | 98.2 % (54 of 55) | 92.6 % (50 of 54) | 0.0 % (0 of 1) |
Methyl | 94.7 % (144 of 152) | 94.7 % (72 of 76) | 94.7 % (72 of 76) |
1. staphylococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDNADSGQTemperature 318 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | staphylococcal nuclease | [U-15N] | 3.5 ~ 5.0 mM | |
2 | THYMIDINE-3',5'-DIPHOSPHATE | 3.5 ~ 5.0 mM | ||
3 | CALCIUM (II) ION | 3.5 ~ 5.0 mM | ||
4 | KCL | 300 mM | ||
5 | D2O | 10 % | ||
6 | H2O | 90 % |