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Overexpression of Myoglobin and Assignment of Its Amide, C Alpha and C Beta Resonances
Authors
Jennings, P.A., Stone, M.J., Wright, P.E.
Assembly
myoglobin carbon monoxide complex
Entity
1. apomyoglobin (polymer), 154 monomers, 17330.85 Da Detail

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG


2. PROTOPORPHYRIN IX (non-polymer), 562.658 Da
3. CARBON MONOXIDE (non-polymer), 28.010 Da
Total weight
17921.518 Da
Max. entity weight
17330.85 Da
Source organism
Physeter catodon
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.4 %, Completeness: 31.5 %, Completeness (bb): 61.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All31.5 % (583 of 1849)16.4 % (157 of 960)38.8 % (282 of 727)88.9 % (144 of 162)
Backbone61.4 % (556 of 905)44.4 % (135 of 304)61.4 % (277 of 451)96.0 % (144 of 150)
Sidechain13.8 % (150 of 1087) 3.4 % (22 of 656)30.5 % (128 of 419) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 156) 0.0 % (0 of 78) 0.0 % (0 of 76) 0.0 % (0 of 2)
Methyl10.3 % (19 of 184) 2.2 % (2 of 92)18.5 % (17 of 92)

1. apomyoglobin

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG

Sample

Temperature 308 K, pH 5.6


#NameIsotope labelingTypeConcentration
1myoglobin carbon monoxide complex[U-90% 15N; U-95% 13C]1 mM
2potassium phosphate0.05 M
3H2O90 %
4D2O10 %

LACS Plot; CA
Referencing offset: 1.73 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 1.73 ppm, Outliers: 1 Detail
Release date
1998-02-24
Citation
Overexpression of myoglobin and assignment of its amide, C alpha and C beta resonances
Jennings, P.A., Stone, M.J., Wright, P.E.
J. Biomol. NMR (1995), 6, 271-276, PubMed 8520219 , DOI 10.1007/bf00197808 ,
Related entities 1. apomyoglobin, : 1 : 13 : 27 : 60 : 101 entities Detail
Interaction partners 1. apomyoglobin, : 2 interactors Detail
Experiments performed 1 experiments Detail
Keywords Heme protein, Heteronuclear NMR, Inclusion bodies, Myoglobin