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1H and 15N Resonance Assignments and Secondary Structure of the Carbon Monoxide Complex of Sperm Whale Myoglobin
Authors
Theriault, Y., Pochapsky, T.C., Dalvit, C., Chiu, M.L., Sligar, S.G., Wright, P.E.
Assembly
myoglobin carbon monoxide complex
Entity
1. apomyoglobin (polymer), 154 monomers, 17330.85 Da Detail

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG


2. PROTOPORPHYRIN IX (non-polymer), 562.658 Da
3. CARBON MONOXIDE (non-polymer), 28.010 Da
Total weight
17921.518 Da
Max. entity weight
17330.85 Da
Source organism
Physeter catodon
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.5 %, Completeness: 75.2 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All75.2 % (844 of 1122)72.6 % (697 of 960)90.7 % (147 of 162)
Backbone94.7 % (430 of 454)94.4 % (287 of 304)95.3 % (143 of 150)
Sidechain62.0 % (414 of 668)62.5 % (410 of 656)33.3 % (4 of 12)
Aromatic100.0 % (80 of 80)100.0 % (78 of 78)100.0 % (2 of 2)
Methyl87.0 % (80 of 92)87.0 % (80 of 92)

1. apomyoglobin

MVLSEGEWQL VLHVWAKVEA DVAGHGQDIL IRLFKSHPET LEKFDRFKHL KTEAEMKASE DLKKHGVTVL TALGAILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISE AIIHVLHSRH PGDFGADAQG AMNKALELFR KDIAAKYKEL GYQG

Sample

Temperature 308 K, pH 5.6


#NameIsotope labelingTypeConcentration
4myoglobin carbon monoxide complex[U-15N]0.0 ~ 0.0 mM
5potassium phosphate0.05 mM
6D2010 %
7H2O90 %

Release date
1997-09-25
Citation
1H and 15N resonance assignments and secondary structure of the carbon monoxide complex of sperm whale myoglobin
Theriault, Y., Pochapsky, T.C., Dalvit, C., Chiu, M.L., Sligar, S.G., Wright, P.E.
J. Biomol. NMR (1994), 4, 491-504, PubMed 8075538 , DOI 10.1007/bf00156616 ,
Related entities 1. apomyoglobin, : 1 : 13 : 27 : 60 : 101 entities Detail
Interaction partners 1. apomyoglobin, : 2 interactors Detail
Experiments performed 1 experiments Detail
Keywords Heme protein, Iron protoporphyrin IX, MbCO, myoglobin complexed with carbon monoxide, NMR