BMRB: 4069

Secondary Structure Determination of 15N-Labelled Human Long-[Arg-3]-Insulin-Like Growth Factor-I by Multidimensional NMR Spectroscopy

Authors

Laajoki, L.G., Le Breton, E., Shooter, G.K., Wallace, J.C., Francis, G.L., Carver, J.A., Keniry, M.A.

Assembly

Long-[Arg-3]-Insulin-Like Growth Factor-I

Entity

1. Long-[Arg-3]-Insulin-Like Growth Factor-I (polymer, Thiol state: all disulfide bound), 83 monomers, 9117.500 Da Detail

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Formula weight

9117.5 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS19:SG	1:CYS61:SG
2	disulfide	sing	1:CYS31:SG	1:CYS74:SG
3	disulfide	sing	1:CYS60:SG	1:CYS65:SG

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.9 %, Completeness (bb): 98.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	94.9 % (543 of 572)	95.1 % (468 of 492)	93.8 % (75 of 80)
Backbone	98.8 % (239 of 242)	98.8 % (164 of 166)	98.7 % (75 of 76)
Sidechain	92.1 % (304 of 330)	93.3 % (304 of 326)	0.0 % (0 of 4)
Aromatic	81.0 % (34 of 42)	81.0 % (34 of 42)
Methyl	100.0 % (36 of 36)	100.0 % (36 of 36)

1. Long-[Arg-3]-Insulin-Like Growth Factor-I

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Show sample condition

Sample

Temperature 303 K, pH 4.1

#	Name	Isotope labeling	Type	Concentration
1	Long-[Arg-3]-Insulin-Like Growth Factor-I	[U-15N]		1.0 mM
2	H2O			90 %
3	D2O			10 %

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Release date

1998-01-08

Citation

Secondary structure determination of 15N-labelled human Long-[Arg-3]-insulin-like growth factor 1 by multidimensional NMR spectroscopy
Laajoki, L.G., Le Breton, E., Shooter, G.K., Wallace, J.C., Francis, G.L., Carver, J.A., Keniry, M.A.
FEBS Lett. (1997), 420, 97-102, PubMed 9450557 , DOI 10.1016/s0014-5793(97)01496-8 , Abstract

Related entities 1. Long-[Arg-3]-Insulin-Like Growth Factor-I, : 1 : 21 : 85 entities Detail

Interaction partners 1. Long-[Arg-3]-Insulin-Like Growth Factor-I, : 9 interactors Detail

Chemical shift validation 3 contents Detail