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Found 1 Document (1.376 seconds)

BMRB: 4070

6E14

Sequence-Specific 1H, 15N and 13C Assignments of the Periplasmic Chaperone FimC from Escherichia coli

Authors

Pellecchia, M., Guntert, P., Glockshuber, R., Wuthrich, K.

Assembly

FimC

Entity

1. FimC (polymer), 205 monomers, 22729.81 Da Detail

GVALGATRVI YPAGQKQEQL AVTNNDENST YLIQSWVENA DGVKDGRFIV TPPLFAMKGK KENTLRILDA TNNQLPQDRE SLFWMNVKAI PSMDKSKLTE NTLQLAIISR IKLYYRPAKL ALPPDQAAEK LRFRRSANSL TLINPTPYYL TVTELNAGTR VLENALVPPM GESTVKLPSD AGSNITYRTI NDYGALTPKM TGVME

Formula weight

22729.81 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.1 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.1 % (2107 of 2392)	86.3 % (1075 of 1246)	88.8 % (826 of 930)	95.4 % (206 of 216)
Backbone	95.4 % (1147 of 1202)	95.6 % (389 of 407)	95.4 % (576 of 604)	95.3 % (182 of 191)
Sidechain	82.9 % (1147 of 1384)	81.8 % (686 of 839)	84.0 % (437 of 520)	96.0 % (24 of 25)
Aromatic	63.3 % (81 of 128)	64.1 % (41 of 64)	61.3 % (38 of 62)	100.0 % (2 of 2)
Methyl	96.1 % (246 of 256)	95.3 % (122 of 128)	96.9 % (124 of 128)

1. chaperone FimC

Show sample condition

Sample #1

Temperature 311 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	chaperone FimC	[U-15N]

Sample #2

Temperature 311 K, pH 5.0

#	Name	Isotope labeling	Concentration
2	chaperone FimC	[U-13C;U-15N]	1 mM
3	H20		90 %
4	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl	null	null	0.2514495
¹H	DSS	methyl	internal	null	0.0
¹⁵N	DSS	methyl	null	null	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl	null	null	0.2514495
¹H	DSS	methyl	internal	null	0.0
¹⁵N	DSS	methyl	null	null	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl	null	null	0.2514495
¹H	DSS	methyl	internal	null	0.0
¹⁵N	DSS	methyl	null	null	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl	null	null	0.2514495
¹H	DSS	methyl	internal	null	0.0
¹⁵N	DSS	methyl	null	null	0.1013291

Referencing offset: 0.63 ppm, Outliers: 2 Detail

Release date

1998-07-14

Citation

Sequence-Specific 1H, 15N and 13C Assignments of the 23 kDa Chaperone FimC from Escherichia coli
Pellecchia, M., Guntert, P., Glockshuber, R., Wuthrich, K.
J. Biomol. NMR (1998), 11, 229-230, PubMed , DOI:

Related entities 1. FimC, : 1 : 16 : 5 : 85 entities Detail

Interaction partners 1. FimC, : 8 interactors Detail

More details for 8 interactors identified by 6 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4070_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4070_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_FimC	Warning	2431		100.0 (chain: 1, length: 205)
1	Chemical shift references	chem_shift_reference_FimC	Warning	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_assignment_FimC

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 205, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GVALGATRVIYPAGQKQEQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GVALGATRVIYPAGQKQEQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTE

-------110-------120-------130-------140-------150-------160-------170-------180-------190-------200
NTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKM
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKM

-----
TGVME
|||||
TGVME

Total number of assignments
- ¹³C chemical shifts: 864
- ¹H chemical shifts: 1340
- ¹⁵N chemical shifts: 227

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
8	ARG	NH1	70.1
8	ARG	HH11	5.88
17	GLN	CD	178.0
18	GLU	CD	180.9
19	GLN	CD	177.8
24	ASN	CG	173.7
25	ASN	CG	175.0
26	ASP	CG	177.9
28	ASN	CG	175.4
34	GLN	CD	177.0
38	GLU	CD	181.4
39	ASN	CG	177.8
41	ASP	CG	178.1
45	ASP	CG	176.3
63	ASN	CG	174.2
66	ARG	HH11	6.73
69	ASP	CG	177.7
72	ASN	CG	175.9
73	ASN	CG	176.6
74	GLN	CD	178.7
77	GLN	CD	178.4
78	ASP	CG	179.1
79	ARG	HH11	6.78
94	ASP	CG	177.7
100	GLU	CD	181.5
101	ASN	CG	174.8
104	GLN	CD	178.1
110	ARG	HH11	6.57
125	ASP	CG	177.8
126	GLN	CD	177.6
129	GLU	CD	180.8
132	ARG	HH11	6.71
134	ARG	HH11	6.72
138	ASN	CG	175.8
154	GLU	CD	181.5
156	ASN	CG	174.5
160	ARG	HH11	6.86
163	GLU	CD	182.4
164	ASN	CG	175.3
172	GLU	CD	180.7
180	ASP	CG	177.3
184	ASN	CG	175.1
191	ASN	CG	174.7
192	ASP	CG	176.7

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	930	828	89.0
¹H chemical shifts	1246	1081	86.8
¹⁵N chemical shifts	227	226	99.6

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	410	394	96.1
¹H chemical shifts	407	405	99.5
¹⁵N chemical shifts	191	190	99.5

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	520	434	83.5
¹H chemical shifts	839	676	80.6
¹⁵N chemical shifts	36	36	100.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	134	126	94.0
¹H chemical shifts	134	126	94.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	62	38	61.3
¹H chemical shifts	64	40	62.5
¹⁵N chemical shifts	2	2	100.0

Peptide bond of PRO
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: 1

Keywords FimC, NMR assignments, periplasmic chaperone, type I pili

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Found 1 Document (1.376 seconds)

BMRB: 4070

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. FimC, : 1 : 16 : 5 : 85 entities Detail

Interaction partners 1. FimC, : 8 interactors Detail

Chemical shift validation 3 contents Detail