BMRBj - BMREntryMaster

Found 1 Document (1.402 seconds)

BMRB: 4091

1BLA

1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy

Authors

Moy, F.J., Seddon, A.P., Campbell, E.B., Bohlen, P., Powers, R.

Assembly

basic fibroblast growth factor

Entity

1. basic fibroblast growth factor (polymer), 154 monomers, 17190.36 Da Detail

AEGEITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVSANR YLAMKEDGRL LASKSVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Formula weight

17190.36 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.8 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.8 % (1752 of 1810)	96.3 % (917 of 952)	97.4 % (685 of 703)	96.8 % (150 of 155)
Backbone	98.8 % (895 of 906)	98.4 % (310 of 315)	99.3 % (443 of 446)	97.9 % (142 of 145)
Sidechain	95.5 % (995 of 1042)	95.3 % (607 of 637)	96.2 % (380 of 395)	80.0 % (8 of 10)
Aromatic	94.4 % (151 of 160)	97.5 % (78 of 80)	91.1 % (72 of 79)	100.0 % (1 of 1)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. basic fibroblast Growth Factor

AEGEITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVSANR YLAMKEDGRL LASKSVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Show sample condition

Sample #1

Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	basic fibroblast Growth Factor	[U-15N]	1 mM
2	DTT		10 mM
3	H2O		90 %
4	D2O		10 %
5	NaN3		2 mM
6	potassium phosphate		50 mM

Sample #2

Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	basic fibroblast Growth Factor	[U-15N; U-13C]	1 mM
8	DTT		10 mM
9	H2O		90 %
10	D2O		10 %
11	NaN3		2 mM
12	potassium phosphate		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	na	external	null
¹H	TSP	na	null	null
¹⁵N	liquid NH3	na	external	null

Referencing offset: 0.28 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	na	external	null
¹H	TSP	na	null	null
¹⁵N	liquid NH3	na	external	null

Referencing offset: 0.28 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	na	external	null
¹H	TSP	na	null	null
¹⁵N	liquid NH3	na	external	null

Referencing offset: 0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	na	external	null
¹H	TSP	na	null	null
¹⁵N	liquid NH3	na	external	null

Referencing offset: 1.0 ppm, Outliers: 1 Detail

Release date

1998-03-13

Citation

1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy
Moy, F.J., Seddon, A.P., Campbell, E.B., Bohlen, P., Powers, R.
J. Biomol. NMR (1995), 6, 245-254, PubMed , DOI:

Related entities 1. basic fibroblast growth factor, : 1 : 1 : 23 : 142 entities Detail

Interaction partners 1. basic fibroblast growth factor, : 14 interactors Detail

More details for 14 interactors identified by 11 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4091_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4091_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment	Warning	1897		100.0 (chain: 1, length: 154)

Assigned chemical shifts

Saveframe: chemical_shift_assignment
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 154, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 686
  - ¹H chemical shifts: 1060
  - ¹⁵N chemical shifts: 151
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	703	686	97.6
¹H chemical shifts	952	918	96.4
¹⁵N chemical shifts	166	151	91.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	308	307	99.7
¹H chemical shifts	315	312	99.0
¹⁵N chemical shifts	145	143	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	395	379	95.9
¹H chemical shifts	637	606	95.1
¹⁵N chemical shifts	21	8	38.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	71	71	100.0
¹H chemical shifts	71	71	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	79	72	91.1
¹H chemical shifts	80	78	97.5
¹⁵N chemical shifts	1	1	100.0

Keywords Basic Fibroblast Growth Factor

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Found 1 Document (1.402 seconds)

BMRB: 4091

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. basic fibroblast growth factor, : 1 : 1 : 23 : 142 entities Detail

Interaction partners 1. basic fibroblast growth factor, : 14 interactors Detail

Chemical shift validation 3 contents Detail