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Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy
Authors
Powers, R., Clore, G.MARIUS., Bax, A., Garrett, D.S., Stahl, S.J., Wingfield, P.T., Gronenborn, A.M.
Assembly
RNase H domain of human HIV-1 Reverse Transcriptase
Entity
1. RNase H domain of human HIV-1 Reverse Transcriptase (polymer), 138 monomers, 15145.01 Da Detail

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAAVPAHKGI GGNEQVDKLV SAGIRKVL


Formula weight
15145.01 Da
Source organism
HIV-1 M:B_HXB2R
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.6 %, Completeness: 40.3 %, Completeness (bb): 74.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.3 % (645 of 1601)32.2 % (267 of 829)42.5 % (261 of 614)74.1 % (117 of 158)
Backbone74.7 % (606 of 811)85.7 % (234 of 273)63.3 % (255 of 403)86.7 % (117 of 135)
Sidechain 5.7 % (52 of 917) 5.9 % (33 of 556) 5.6 % (19 of 338) 0.0 % (0 of 23)
Aromatic 0.0 % (0 of 62) 0.0 % (0 of 31) 0.0 % (0 of 31)
Methyl 7.9 % (15 of 190) 7.4 % (7 of 95) 8.4 % (8 of 95)

1. Reverse Transcriptase of HIV-1

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAAVPAHKGI GGNEQVDKLV SAGIRKVL

Sample

Temperature 299 K, pH 5.4


#NameIsotope labelingTypeConcentration
1Reverse Transcriptase of HIV-1[U-95% 13C; U-95% 15N]1.1 mM
2H2O90 %
3D2O10 %
4Sodium phosphate buffer100 mM

Release date
1998-03-01
Citation
Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy
Powers, R., Clore, G.MARIUS., Bax, A., Garrett, D.S., Stahl, S.J., Wingfield, P.T., Gronenborn, A.M.
J. Mol. Biol. (1991), 221, 1081-1090, PubMed 1719214 ,
Related entities 1. RNase H domain of human HIV-1 Reverse Transcriptase, : 1 : 96 : 97 entities Detail
Interaction partners 1. RNase H domain of human HIV-1 Reverse Transcriptase, : 5 interactors Detail
Keywords 3D heteronuclear NMR, Double and triple resonance NMR, HIV-1, Reverse Transcriptase, RNase H domain (RNase H)