BMRB: 4093

Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy

Authors

Powers, R., Clore, G.MARIUS., Bax, A., Garrett, D.S., Stahl, S.J., Wingfield, P.T., Gronenborn, A.M.

Assembly

RNase H domain of human HIV-1 Reverse Transcriptase

Entity

1. RNase H domain of human HIV-1 Reverse Transcriptase (polymer), 138 monomers, 15145.01 Da Detail

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAAVPAHKGI GGNEQVDKLV SAGIRKVL

Formula weight

15145.01 Da

Source organism

HIV-1 M:B_HXB2R

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.6 %, Completeness: 40.2 %, Completeness (bb): 74.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	40.2 % (642 of 1597)	31.8 % (264 of 829)	42.3 % (260 of 614)	76.6 % (118 of 154)
Backbone	74.8 % (615 of 822)	86.3 % (245 of 284)	62.5 % (252 of 403)	87.4 % (118 of 135)
Sidechain	4.1 % (37 of 902)	3.5 % (19 of 545)	5.3 % (18 of 338)	0.0 % (0 of 19)
Aromatic	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)
Methyl	6.3 % (12 of 190)	5.3 % (5 of 95)	7.4 % (7 of 95)

1. Reverse Transcriptase of HIV-1

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAAVPAHKGI GGNEQVDKLV SAGIRKVL

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Sample

Temperature 299 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	Reverse Transcriptase of HIV-1	[U-95% 13C; U-95% 15N]		1.1 mM
2	H2O			90 %
3	D2O			10 %
4	Sodium phosphate buffer			100 mM

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Release date

1998-03-01

Citation

Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy
Powers, R., Clore, G.MARIUS., Bax, A., Garrett, D.S., Stahl, S.J., Wingfield, P.T., Gronenborn, A.M.
J. Mol. Biol. (1991), 221, 1081-1090, PubMed 1719214 , Abstract

Related entities 1. RNase H domain of human HIV-1 Reverse Transcriptase, : 1 : 96 : 115 entities Detail

Interaction partners 1. RNase H domain of human HIV-1 Reverse Transcriptase, : 5 interactors Detail

Chemical shift validation 3 contents Detail