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1H, 13C and 15N Chemical Shift Assignments of the 14mer DNA Duplex BS2 in the Complex with the Antennapedia Homeodomain
Authors
Fernandez, C., Szyperski, T., Ono, A., Iwai, H., Tate, S., Kainosho, M., Wuthrich, K.
Assembly
BS2 operator DNA complexed with the Antennapedia Homeodomain
Entity
1. BS2 strand A (polymer), 14 monomers, 4400.827 Da Detail

GAAAGCCATT AGAG


2. BS2 strand B (polymer), 14 monomers, 4284.723 Da Detail

CTCTAATGGC TTTC


3. Antennapedia Homeodomain (polymer), 68 monomers, 8638.914 Da Detail

MRKRGRQTYT RYQTLELEKE FHFNRYLTRR RRIEIAHALS LTERQIKIWF QNRRMKWKKE NKTKGEPG


Total weight
17324.465 Da
Max. entity weight
8638.914 Da
Source organism
Drosophila melanogaster
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 17.7 %, Completeness: 11.8 %, Completeness (bb): 17.7 % Detail

Polymer type: polydeoxyribonucleotide polypeptide(L)

Total1H13C15N
All11.8 % (141 of 1190)16.8 % (128 of 762) 2.6 % (9 of 340) 4.5 % (4 of 88)
Backbone17.7 % (106 of 599)29.3 % (97 of 331) 3.0 % (6 of 201) 4.5 % (3 of 67)
Sidechain 5.6 % (37 of 656) 7.2 % (31 of 431) 2.5 % (5 of 204) 4.8 % (1 of 21)
Aromatic18.3 % (26 of 142)26.3 % (26 of 99) 0.0 % (0 of 41) 0.0 % (0 of 2)
Methyl 0.0 % (0 of 60) 0.0 % (0 of 34) 0.0 % (0 of 26)

1. BS2 strand A

GAAAGCCATT AGAG

2. BS2 strand B

CTCTAATGGC TTTC

3. Antennapedia Homeodomain

MRKRGRQTYT RYQTLELEKE FHFNRYLTRR RRIEIAHALS LTERQIKIWF QNRRMKWKKE NKTKGEPG

Sample

Temperature 309 K, pH 6.0


#NameIsotope labelingTypeConcentration
1BS2_strand_A[U-13C;U-15N]1.0 mM
2BS2_strand_B[U-13C;U-15N]1.0 mM
3Antennapedia Homeodomain[U-15N]1.0 mM
4D2O10 %
5H2O90 %

Release date
1998-07-13
Citation
NMR with 13C,15N-doubly-labeled DNA: the Antennapedia Homeodomain Complex with a 14mer DNA Duplex
Fernandez, C., Szyperski, T., Ono, A., Iwai, H., Tate, S., Kainosho, M., Wuthrich, K.
J. Biomol. NMR (1998)
Related entities 3. Antennapedia Homeodomain, : 1 : 1 : 12 : 172 entities Detail
Interaction partners 3. Antennapedia Homeodomain, : 9 interactors Detail
Experiments performed 1 experiments Detail
Keywords isotope labeling, NMR spectroscopy, Nucleic acids, protein-DNA complex, sequential resonance assignment