BMRB: 4104

1H, 13C and 15N Chemical Shift Assignments of the 14mer DNA Duplex BS2 in the Complex with the Antennapedia Homeodomain

Authors

Fernandez, C., Szyperski, T., Ono, A., Iwai, H., Tate, S., Kainosho, M., Wuthrich, K.

Assembly

BS2 operator DNA complexed with the Antennapedia Homeodomain

Entity

1. BS2 strand A (polymer), 14 monomers, 4400.827 Da Detail

GAAAGCCATT AGAG

2. BS2 strand B (polymer), 14 monomers, 4284.723 Da Detail

CTCTAATGGC TTTC

3. Antennapedia Homeodomain (polymer), 68 monomers, 8638.914 Da Detail

MRKRGRQTYT RYQTLELEKE FHFNRYLTRR RRIEIAHALS LTERQIKIWF QNRRMKWKKE NKTKGEPG

Total weight

17324.465 Da

Max. entity weight

8638.914 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 15.6 %, Completeness: 11.8 %, Completeness (bb): 16.6 % Detail

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Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	1H	13C	15N
All	11.8 % (139 of 1178)	17.3 % (132 of 762)	1.8 % (6 of 340)	1.3 % (1 of 76)
Backbone	16.6 % (100 of 602)	28.7 % (96 of 334)	1.5 % (3 of 201)	1.5 % (1 of 67)
Sidechain	6.2 % (40 of 641)	8.4 % (36 of 428)	2.0 % (4 of 204)	0.0 % (0 of 9)
Aromatic	18.3 % (26 of 142)	26.3 % (26 of 99)	0.0 % (0 of 41)	0.0 % (0 of 2)
Methyl	0.0 % (0 of 60)	0.0 % (0 of 34)	0.0 % (0 of 26)

1. BS2 strand A

GAAAGCCATT AGAG

2. BS2 strand B

CTCTAATGGC TTTC

3. Antennapedia Homeodomain

MRKRGRQTYT RYQTLELEKE FHFNRYLTRR RRIEIAHALS LTERQIKIWF QNRRMKWKKE NKTKGEPG

Show sample condition

Sample

Temperature 309 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	BS2_strand_A	[U-13C;U-15N]		1.0 mM
2	BS2_strand_B	[U-13C;U-15N]		1.0 mM
3	Antennapedia Homeodomain	[U-15N]		1.0 mM
4	D2O			10 %
5	H2O			90 %

Hide sample codition

Release date

1998-07-13

Citation

NMR with 13C,15N-doubly-labeled DNA: the Antennapedia Homeodomain Complex with a 14mer DNA Duplex
Fernandez, C., Szyperski, T., Ono, A., Iwai, H., Tate, S., Kainosho, M., Wuthrich, K.
J. Biomol. NMR (1998), PubMed

Related entities 3. Antennapedia Homeodomain, : 1 : 1 : 13 : 271 entities Detail

Interaction partners 3. Antennapedia Homeodomain, : 9 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 5 contents Detail