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Sequence Specific 1H, 13C and 15N Resonance Assignment of Rat CD2 Domain 1
Authors
Chen, H.A., Pfuhl, M., Davis, B., Driscoll, P.C.
Assembly
rat CD2 domain 1
Entity
1. rat CD2 domain 1 (polymer), 100 monomers, 11273.72 Da Detail

MRDSGTVWGA LGHGINLNIP NFQMTDDIDE VRWERGSTLV AEFKRKMKPF LKSGAFEILA NGDLKIKNLT RDDSGTYNVT VYSTNGTRIL NKALDLRILE


Formula weight
11273.72 Da
Source organism
Rattus rattus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 77.3 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All77.3 % (911 of 1179)82.1 % (500 of 609)69.2 % (314 of 454)83.6 % (97 of 116)
Backbone82.1 % (482 of 587)94.4 % (187 of 198)68.4 % (199 of 291)98.0 % (96 of 98)
Sidechain75.0 % (512 of 683)76.2 % (313 of 411)78.0 % (198 of 254) 5.6 % (1 of 18)
Aromatic 0.0 % (0 of 84) 0.0 % (0 of 42) 0.0 % (0 of 40) 0.0 % (0 of 2)
Methyl98.3 % (116 of 118)98.3 % (58 of 59)98.3 % (58 of 59)

1. rat CD2 domain 1

MRDSGTVWGA LGHGINLNIP NFQMTDDIDE VRWERGSTLV AEFKRKMKPF LKSGAFEILA NGDLKIKNLT RDDSGTYNVT VYSTNGTRIL NKALDLRILE

Sample

Temperature 298 (±0.1) K, pH 3.0 (±0.03)


#NameIsotope labelingTypeConcentration
1rat CD2 domain 1[U96%-13C;U96%-15N]1.2 mM
2phosphate buffer20 mM

Chem. Shift Complete2
Sequence coverage: 99.0 %, Completeness: 86.8 %, Completeness (bb): 90.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.8 % (2047 of 2358)89.7 % (1092 of 1218)81.8 % (743 of 908)91.4 % (212 of 232)
Backbone90.5 % (1063 of 1174)96.7 % (383 of 396)83.7 % (487 of 582)98.5 % (193 of 196)
Sidechain84.7 % (1157 of 1366)86.3 % (709 of 822)84.4 % (429 of 508)52.8 % (19 of 36)
Aromatic35.1 % (59 of 168)41.7 % (35 of 84)27.5 % (22 of 80)50.0 % (2 of 4)
Methyl99.2 % (234 of 236)99.2 % (117 of 118)99.2 % (117 of 118)

1. rat CD2 domain 1

MRDSGTVWGA LGHGINLNIP NFQMTDDIDE VRWERGSTLV AEFKRKMKPF LKSGAFEILA NGDLKIKNLT RDDSGTYNVT VYSTNGTRIL NKALDLRILE

Sample

Temperature 298 (±0.1) K, pH 5.0 (±0.03)


#NameIsotope labelingTypeConcentration
1rat CD2 domain 1[U96%-13C;U96%-15N]1.2 mM
2phosphate buffer20 mM

Chem. Shift Complete3
Sequence coverage: 99.0 %, Completeness: 82.9 %, Completeness (bb): 86.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All82.9 % (2932 of 3537)86.8 % (1586 of 1827)76.4 % (1041 of 1362)87.6 % (305 of 348)
Backbone86.5 % (1524 of 1761)95.8 % (569 of 594)77.0 % (672 of 873)96.3 % (283 of 294)
Sidechain81.1 % (1662 of 2049)82.5 % (1017 of 1233)81.8 % (623 of 762)40.7 % (22 of 54)
Aromatic25.8 % (65 of 252)30.2 % (38 of 126)20.0 % (24 of 120)50.0 % (3 of 6)
Methyl99.4 % (352 of 354)99.4 % (176 of 177)99.4 % (176 of 177)

1. rat CD2 domain 1

MRDSGTVWGA LGHGINLNIP NFQMTDDIDE VRWERGSTLV AEFKRKMKPF LKSGAFEILA NGDLKIKNLT RDDSGTYNVT VYSTNGTRIL NKALDLRILE

Sample

Temperature 298 (±0.1) K, pH 7.5 (±0.03)


#NameIsotope labelingTypeConcentration
1rat CD2 domain 1[U96%-13C;U96%-15N]1.2 mM
2phosphate buffer20 mM

LACS Plot; CA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.01 ppm, Outliers: 2 Detail
Release date
1999-02-01
Citation 1
Sequence Specific 1H, 13C and 15N Resonance Assignment of Rat CD2 Domain 1
Chen, H.A., Pfuhl, M., Davis, B., Driscoll, P.C.
J. Biomol. NMR (1998), 12, 457-458, PubMed , DOI:
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 ,
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Related entities 1. rat CD2 domain 1, : 1 : 19 entities Detail
Interaction partners 1. rat CD2 domain 1, : 1 interactors Detail
Experiments performed 10 experiments Detail
Keywords CD2, CD48, cell adhesion molecules, T lymphocyte