BMRB: 4152

1H, 13C and 15N Backbone Resonance Assignment of Escherichia coli Adenylate Kinase, a 23.6 kDa Protein

Authors

Burlacu-Miron, S., Perrier, V., Gilles, A., Mispelter, J., Barzu, O., Craescu, C.T.

Assembly

Adenylate kinase from E. coli

Entity

1. Adenylate kinase from E. coli (polymer, Thiol state: not reported), 214 monomers, 23585.72 Da Detail

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Formula weight

23585.72 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 60.4 %, Completeness (bb): 80.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	60.4 % (1483 of 2456)	66.1 % (847 of 1282)	45.3 % (434 of 958)	93.5 % (202 of 216)
Backbone	80.0 % (1011 of 1264)	93.2 % (408 of 438)	65.3 % (406 of 622)	96.6 % (197 of 204)
Sidechain	46.8 % (649 of 1386)	52.0 % (439 of 844)	38.7 % (205 of 530)	41.7 % (5 of 12)
Aromatic	37.3 % (44 of 118)	74.6 % (44 of 59)	0.0 % (0 of 59)
Methyl	44.5 % (114 of 256)	64.1 % (82 of 128)	25.0 % (32 of 128)

1. Adenylate kinase

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Show sample condition

Sample #1

Temperature 308 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	Adenylate kinase			1.2 mM
2	H2O			93 %
3	D2O			7 %

Sample #2

Temperature 308 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
4	Adenylate kinase	[U-15N]		1.0 mM
5	H2O			93 %
6	D2O			7 %

Sample #3

Temperature 308 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
7	Adenylate kinase	[U-13C; U15-N]		1.0 mM
8	H2O			93 %
9	D2O			7 %

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LACS Plot; CA

Referencing offset: 2.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbon	67.8 ppm	external	indirect	0.0
1H	H2O	proton	4.68 ppm	internal	direct	0.0
15N	liquid ammonia	nitrogen	0.0 ppm	external	null	0.0

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LACS Plot; CB

Referencing offset: 2.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbon	67.8 ppm	external	indirect	0.0
1H	H2O	proton	4.68 ppm	internal	direct	0.0
15N	liquid ammonia	nitrogen	0.0 ppm	external	null	0.0

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbon	67.8 ppm	external	indirect	0.0
1H	H2O	proton	4.68 ppm	internal	direct	0.0
15N	liquid ammonia	nitrogen	0.0 ppm	external	null	0.0

Hide chemical shift referencing parameters

Release date

2000-03-01

Citation

1H, 13C and 15N backbone resonance assignment of Escherichia coli adenylate kinase, a 23.6 kDa protein
Burlacu-Miron, S., Perrier, V., Gilles, A., Mispelter, J., Barzu, O., Craescu, C.T.
J. Biomol. NMR (1999), 13, 93-94, PubMed 10070751 , Abstract

Related entities 1. Adenylate kinase from E. coli, : 1 : 11 : 3 : 14 : 269 entities Detail

Interaction partners 1. Adenylate kinase from E. coli, : 11 interactors Detail

Chemical shift validation 3 contents Detail